5OMF

Closed, ternary structure of KOD DNA polymerase

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	DNA polymerase,DNA polymerase,DNA polymerase	polymer	774	90060.5	1	UniProt (P77933) Pfam (PF03104) Pfam (PF00136)	Thermococcus kodakarensis (strain ATCC BAA-918 / JCM 12380 / KOD1)
2	B (T)	DNA (5'-D(P*CP*TP*GP*TP*GP*GP*CP*CP*GP*TP*GP*GP*TP*C)-3')	polymer	16	4930.2	1		synthetic construct
3	C (P)	DNA (5'-D(*GP*AP*CP*CP*AP*CP*GP*GP*CP*CP*AP*C)-3')	polymer	16	4868.2	1		synthetic construct
4	D, E (A)	MANGANESE (II) ION	non-polymer		54.9	2	Chemie (MN)
5	F, G, H, I (A)	1,2-ETHANEDIOL	non-polymer		62.1	4	Chemie (EDO)
6	J (A)	MAGNESIUM ION	non-polymer		24.3	1	Chemie (MG)
7	K, L, N (A, P)	GLYCEROL	non-polymer		92.1	3	Chemie (GOL)
8	M (A)	2'-DEOXYADENOSINE 5'-TRIPHOSPHATE	non-polymer		491.2	1	Chemie (DTP)
9	O, P, Q (A, T, P)	water	water		18.0	269	Chemie (HOH)

Sequence modifications

A: 1 - 406 (UniProt: P77933)

PDB	External Database	Details
Ala 141	Asp 141	engineered mutation
Ala 143	Glu 143	engineered mutation

A: 407 - 491 (UniProt: P77933)
A: 492 - 774 (UniProt: P77933)

Contents of the asymmetric unit

Polymers	Number of chains	3
	Total formula weight	99858.8
Non-Polymers*	Number of molecules	11
	Total formula weight	1149.9
All*	Total formula weight	101008.7

*Water molecules are not included.