5OMF

Closed, ternary structure of KOD DNA polymerase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-08-10
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9999766
Spacegroup name	P 21 21 2
Unit cell lengths	107.909, 147.559, 71.142
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.266 - 2.092
R-factor	0.1973
Rwork	0.195
R-free	0.23350
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4k8z
RMSD bond length	0.002
RMSD bond angle	0.527
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX ((1.12rc1_2815: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.190	2.220
High resolution limit [Å]	2.090	2.090
Rmeas	0.185	1.720
Number of reflections	127923
<I/σ(I)>	6.37	0.81
Completeness [%]	98.8	95.8
Redundancy	3.56	3.41
CC(1/2)	0.993	0.285

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	291	0.2 M DL-glutamatic acid, 0.2 M DL-alanine, 0.2 M glycine, 0.2 M DL-lysine monohydrochloride, 0.2 M DL-serine, 0.1 M Hepes/ 0.1 M Mops, 20 % glycerol, 10 % PEG 4000