5OKZ

Crystal Strucrure of the Mpp6 Exosome complex

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, DA, J, T (m, I, S, c)	Exosome complex component CSL4	polymer	295	31911.6	4	UniProt (P53859) Pfam (PF21551) Pfam (PF10447)	Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)	CEP1 synthetic lethal protein 4
2	B, FA, L, V (A, K, U, e)	Exosome complex component RRP45	polymer	305	34001.9	4	UniProt (Q05636) Pfam (PF01138) Pfam (PF03725)	Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)	Ribosomal RNA-processing protein 45
3	C, GA, M, W (B, L, V, f)	Exosome complex component SKI6	polymer	249	27892.0	4	UniProt (P46948) Pfam (PF01138) Pfam (PF03725)	Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)	Extracellular mutant protein 20,Ribosomal RNA-processing protein 41,Superkiller protein 6
4	D, HA, N, X (C, M, W, g)	Exosome complex component RRP43	polymer	394	44093.0	4	UniProt (P25359) Pfam (PF01138)	Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)	Ribosomal RNA-processing protein 43
5	E, IA, O, Y (D, N, X, h)	Exosome complex component RRP46	polymer	226	24659.4	4	UniProt (P53256) Pfam (PF01138) Pfam (PF03725)	Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)	Ribosomal RNA-processing protein 46
6	F, JA, P, Z (E, O, Y, i)	Exosome complex component RRP42	polymer	268	29391.5	4	UniProt (Q12277)	Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)	Ribosomal RNA-processing protein 42
7	AA, G, KA, Q (F, P, Z, j)	Exosome complex component MTR3	polymer	250	27573.9	4	UniProt (P48240) Pfam (PF01138)	Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)	mRNA transport regulator 3
8	BA, H, LA, R (G, Q, a, k)	Exosome complex component RRP40	polymer	244	26939.7	4	UniProt (Q08285) Pfam (PF21262) Pfam (PF15985) Pfam (PF18311)	Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)	Ribosomal RNA-processing protein 40
9	CA, I, MA, S (H, R, b, l)	Exosome complex component RRP4	polymer	316	34603.1	4	UniProt (P38792) Pfam (PF14382) Pfam (PF21266) Pfam (PF15985)	Saccharomyces cerevisiae (Baker's yeast)	Ribosomal RNA-processing protein 4
10	EA, K, NA, U (J, T, d, n)	M-phase phosphoprotein 6 homolog	polymer	190	21541.9	4	UniProt (P53725) Pfam (PF10175)	Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)	Exosome-associated RNA-binding protein MPP6
11	OA, RA, VA, XA (A, K, U, e)	TRIS(HYDROXYETHYL)AMINOMETHANE	non-polymer		163.2	4	Chemie (TAM)
12	AB, PA, QA, TA, UA... (l, G, H, R, b...)	CHLORIDE ION	non-polymer		35.5	7	Chemie (CL)
13	SA, ZA (O, g)	MAGNESIUM ION	non-polymer		24.3	2	Chemie (MG)
14	AC, BB, CB, DB, EB... (l, A, B, C, D...)	water	water		18.0	140	Chemie (HOH)

Sequence modifications

m, I, S, c: 1 - 292 (UniProt: P53859)

PDB	External Database	Details
Gly -2	-	expression tag
Pro -1	-	expression tag
His 0	-	expression tag

B, L, V, f: 1 - 246 (UniProt: P46948)

PDB	External Database	Details
Gly -2	-	expression tag
Pro -1	-	expression tag
His 0	-	expression tag

C, M, W, g: 1 - 394 (UniProt: P25359)

PDB	External Database	Details
Met 363	Val 363	conflict

D, N, X, h: 1 - 223 (UniProt: P53256)

PDB	External Database	Details
Ala -2	-	expression tag
Ala -1	-	expression tag
Ser 0	-	expression tag

E, O, Y, i: 1 - 265 (UniProt: Q12277)

PDB	External Database	Details
Gly -2	-	expression tag
Pro -1	-	expression tag
His 0	-	expression tag
Ile 138	Val 138	conflict

F, P, Z, j: 1 - 250 (UniProt: P48240)

PDB	External Database	Details
Thr 161	Met 161	conflict

G, Q, a, k: 1 - 240 (UniProt: Q08285)

PDB	External Database	Details
Gly -3	-	expression tag
Pro -2	-	expression tag
Asp -1	-	expression tag
Ser 0	-	expression tag

H, R, b, l: 50 - 359 (UniProt: P38792)

PDB	External Database	Details
Thr 44	-	expression tag
Gly 45	-	expression tag
Gly 46	-	expression tag
Arg 47	-	expression tag
Ser 48	-	expression tag
Met 49	-	expression tag

J, T, d, n: 1 - 186 (UniProt: P53725)

PDB	External Database	Details
Gly -3	-	expression tag
Pro -2	-	expression tag
Asp -1	-	expression tag
Ser 0	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	40
	Total formula weight	1210432.2
Non-Polymers*	Number of molecules	13
	Total formula weight	949.6
All*	Total formula weight	1211381.8

*Water molecules are not included.