5OKZ
Crystal Strucrure of the Mpp6 Exosome complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X10SA |
Synchrotron site | SLS |
Beamline | X10SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-10-10 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 1.25 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 161.779, 237.430, 201.895 |
Unit cell angles | 90.00, 110.37, 90.00 |
Refinement procedure
Resolution | 80.760 - 3.200 |
R-factor | 0.224490359891 |
Rwork | 0.223 |
R-free | 0.26152 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5jea |
Data reduction software | Aimless |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX (1.10.1_2155) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 151.660 | 3.250 |
High resolution limit [Å] | 3.200 | 3.200 |
Rmerge | 0.214 | 1.881 |
Rpim | 0.114 | 1.298 |
Number of reflections | 229497 | |
<I/σ(I)> | 5.7 | 0.6 |
Completeness [%] | 97.7 | 79.9 |
Redundancy | 4.3 | 2.8 |
CC(1/2) | 0.985 | 0.243 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 291 | 0.1 M Tris/Mops pH 7.5 30 mM MgCl2, 30 mM CaCl2 30% PEG 8000/Ethylene glycol |