5OJM
Structure of a chimaeric beta3-alpha5 GABAA receptor in complex with nanobody Nb25
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B, C, D, E (A, B, C, D, E) | Human GABAA receptor chimera beta3-alpha5,Gamma-aminobutyric acid receptor subunit beta-3,Gamma-aminobutyric acid receptor subunit alpha-5 | polymer | 395 | 44433.4 | 5 | UniProt (P28472) UniProt (P31644) Pfam (PF02931) Pfam (PF02932) | synthetic construct | GABA(A) receptor subunit beta-3,GABA(A) receptor subunit alpha-5 |
| 2 | F, G, H, I, J (K, L, M, N, O) | Nanobody Nb25 | polymer | 125 | 13673.0 | 5 | Lama glama (Llama) | ||
| 3 | K, L, M (F, G, H) | alpha-D-mannopyranose-(1-3)-[alpha-D-mannopyranose-(1-6)]beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose | branched | 910.8 | 3 | In PDB GlyTouCan (G22768VO) | |||
| 4 | N, O (I, J) | alpha-D-mannopyranose-(1-3)-alpha-D-mannopyranose-(1-6)-[alpha-D-mannopyranose-(1-3)]beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose | branched | 1073.0 | 2 | In PDB GlyTouCan (G09724ZC) | |||
| 5 | P, Q, R, S, T (A, B, C, D, E) | 2-acetamido-2-deoxy-beta-D-glucopyranose | non-polymer | 221.2 | 5 | Chemie (NAG) |
Sequence modifications
A, B, C, D, E: -30 - 0 (PDB: 5OJM)
A, B, C, D, E: 1 - 217 (UniProt: P28472)
A, B, C, D, E: 226 - 315 (UniProt: P31644)
A, B, C, D, E: 393 - 431 (UniProt: P31644)
A, B, C, D, E: 1 - 217 (UniProt: P28472)
A, B, C, D, E: 226 - 315 (UniProt: P31644)
A, B, C, D, E: 393 - 431 (UniProt: P31644)
| PDB | External Database | Details |
|---|---|---|
| Ser 316 | - | linker |
| Gln 317 | - | linker |
| Pro 318 | - | linker |
| Ala 319 | - | linker |
| Arg 320 | - | linker |
| Ala 321 | - | linker |
| Ala 322 | - | linker |
| Ile 404 | Val 435 | conflict |
| Thr 428 | Ala 459 | conflict |
| Gly 432 | - | expression tag |
| Thr 433 | - | expression tag |
| Thr 434 | - | expression tag |
| Glu 435 | - | expression tag |
| Thr 436 | - | expression tag |
| Ser 437 | - | expression tag |
| Gln 438 | - | expression tag |
| Val 439 | - | expression tag |
| Ala 440 | - | expression tag |
| Pro 441 | - | expression tag |
| Ala 442 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 10 |
| Total formula weight | 290532.0 | |
| Branched | Number of molecules | 5 |
| Total formula weight | 4878.4 | |
| Non-Polymers* | Number of molecules | 5 |
| Total formula weight | 1106.0 | |
| All* | Total formula weight | 296516.4 |
