5OJM

Structure of a chimaeric beta3-alpha5 GABAA receptor in complex with nanobody Nb25

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04
Synchrotron site	Diamond
Beamline	I04
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-11-26
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.9795
Spacegroup name	C 1 2 1
Unit cell lengths	177.350, 139.940, 191.500
Unit cell angles	90.00, 102.25, 90.00

Refinement procedure

Resolution	48.880 - 3.300
R-factor	0.23449
Rwork	0.234
R-free	0.24960
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4cof
RMSD bond length	0.009
RMSD bond angle	0.710
Data reduction software	HKL-2000 (708c)
Data scaling software	Aimless (0.5.25)
Phasing software	PHASER (2.5.7)
Refinement software	REFMAC (5.8.0158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.880	3.418
High resolution limit [Å]	3.300	3.300
Rmerge	0.352	2.807
Rpim	0.116	0.935
Number of reflections	68852	6886
<I/σ(I)>	7.85	1.08
Completeness [%]	99.8	99.71
Redundancy	10	9.9
CC(1/2)	0.998	0.395

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.8	277.15	14% poly-ethylene glycol (PEG) 5000, 0.08M magnesium acetate, 0.1M sodium citrate, 1mM ethylene glycol-bis(beta-aminoethyl ether)-N,N,N',N'-tetraacetic acid (EGTA)