5OHS
A GH31 family sulfoquinovosidase mutant D455N in complex with pNPSQ
Entity
Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
1 | A, B, C, D, E... | Alpha-glucosidase yihQ | polymer | 672 | 75231.4 | 8 | UniProt (A0A083ZKV2) Pfam (PF01055) Pfam (PF21365) UniProt (by SIFTS) (A9CEZ0) In PDB | Rhizobium radiobacter (Agrobacterium tumefaciens) | |
2 | D, H, E, A, F... | THIOCYANATE ION | non-polymer | 58.1 | 8 | Chemie (SCN) | |||
3 | D, E, A, F, B | (4S)-2-METHYL-2,4-PENTANEDIOL | non-polymer | 118.2 | 9 | Chemie (MPD) | |||
4 | H, E, A, F, B... | CHLORIDE ION | non-polymer | 35.5 | 6 | Chemie (CL) | |||
5 | G, D, H, E, A... | 1,2-ETHANEDIOL | non-polymer | 62.1 | 18 | Chemie (EDO) | |||
6 | G, D, H, E, A... | 4-nitrophenyl alpha-D-6-sulfoquinovoside | non-polymer | 365.3 | 8 | Chemie (NSQ) | |||
7 | C, D | POTASSIUM ION | non-polymer | 39.1 | 2 | Chemie (K) | |||
8 | water | water | 18.0 | 2168 | Chemie (HOH) |
Sequence modifications
A, B, C, D, E, F, G, H: 1 - 664 (UniProt: A0A083ZKV2)
PDB | External Database | Details |
---|---|---|
Ala 370 | Glu 370 | engineered mutation |
Ala 371 | Glu 371 | engineered mutation |
Asn 455 | Asp 455 | engineered mutation |
Leu 665 | - | expression tag |
Glu 666 | - | expression tag |
His 667 | - | expression tag |
His 668 | - | expression tag |
His 669 | - | expression tag |
His 670 | - | expression tag |
His 671 | - | expression tag |
His 672 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
Polymers | Number of chains | 8 |
Total formula weight | 601851.5 | |
Non-Polymers* | Number of molecules | 51 |
Total formula weight | 5858.9 | |
All* | Total formula weight | 607710.4 |