5OHS

A GH31 family sulfoquinovosidase mutant D455N in complex with pNPSQ

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0003824	molecular_function	catalytic activity
A	0004553	molecular_function	hydrolase activity, hydrolyzing O-glycosyl compounds
A	0005975	biological_process	carbohydrate metabolic process
A	0016798	molecular_function	hydrolase activity, acting on glycosyl bonds
A	0030246	molecular_function	carbohydrate binding
B	0003824	molecular_function	catalytic activity
B	0004553	molecular_function	hydrolase activity, hydrolyzing O-glycosyl compounds
B	0005975	biological_process	carbohydrate metabolic process
B	0016798	molecular_function	hydrolase activity, acting on glycosyl bonds
B	0030246	molecular_function	carbohydrate binding
C	0003824	molecular_function	catalytic activity
C	0004553	molecular_function	hydrolase activity, hydrolyzing O-glycosyl compounds
C	0005975	biological_process	carbohydrate metabolic process
C	0016798	molecular_function	hydrolase activity, acting on glycosyl bonds
C	0030246	molecular_function	carbohydrate binding
D	0003824	molecular_function	catalytic activity
D	0004553	molecular_function	hydrolase activity, hydrolyzing O-glycosyl compounds
D	0005975	biological_process	carbohydrate metabolic process
D	0016798	molecular_function	hydrolase activity, acting on glycosyl bonds
D	0030246	molecular_function	carbohydrate binding
E	0003824	molecular_function	catalytic activity
E	0004553	molecular_function	hydrolase activity, hydrolyzing O-glycosyl compounds
E	0005975	biological_process	carbohydrate metabolic process
E	0016798	molecular_function	hydrolase activity, acting on glycosyl bonds
E	0030246	molecular_function	carbohydrate binding
F	0003824	molecular_function	catalytic activity
F	0004553	molecular_function	hydrolase activity, hydrolyzing O-glycosyl compounds
F	0005975	biological_process	carbohydrate metabolic process
F	0016798	molecular_function	hydrolase activity, acting on glycosyl bonds
F	0030246	molecular_function	carbohydrate binding
G	0003824	molecular_function	catalytic activity
G	0004553	molecular_function	hydrolase activity, hydrolyzing O-glycosyl compounds
G	0005975	biological_process	carbohydrate metabolic process
G	0016798	molecular_function	hydrolase activity, acting on glycosyl bonds
G	0030246	molecular_function	carbohydrate binding
H	0003824	molecular_function	catalytic activity
H	0004553	molecular_function	hydrolase activity, hydrolyzing O-glycosyl compounds
H	0005975	biological_process	carbohydrate metabolic process
H	0016798	molecular_function	hydrolase activity, acting on glycosyl bonds
H	0030246	molecular_function	carbohydrate binding

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	8
Details	ACT_SITE: Nucleophile => ECO:0000250|UniProtKB:P32138

Chain	Residue	Details
A	ASP388
B	ASP388
C	ASP388
D	ASP388
E	ASP388
F	ASP388
G	ASP388
H	ASP388

---

site_id	SWS_FT_FI2
Number of Residues	8
Details	ACT_SITE: ACT_SITE => ECO:0000250|UniProtKB:P31434

Chain	Residue	Details
A	GLU391
B	GLU391
C	GLU391
D	GLU391
E	GLU391
F	GLU391
G	GLU391
H	GLU391

---

site_id	SWS_FT_FI3
Number of Residues	8
Details	ACT_SITE: Proton donor => ECO:0000250|UniProtKB:P32138

Chain	Residue	Details
A	ASN455
B	ASN455
C	ASN455
D	ASN455
E	ASN455
F	ASN455
G	ASN455
H	ASN455

---

site_id	SWS_FT_FI4
Number of Residues	16
Details	BINDING: BINDING => ECO:0000269|PubMed:35074914, ECO:0007744|PDB:7OFX

Chain	Residue	Details
A	GLU135
E	ARG438
F	GLU135
F	ARG438
G	GLU135
G	ARG438
H	GLU135
H	ARG438
A	ARG438
B	GLU135
B	ARG438
C	GLU135
C	ARG438
D	GLU135
D	ARG438
E	GLU135

---

site_id	SWS_FT_FI5
Number of Residues	40
Details	BINDING: BINDING => ECO:0000269|PubMed:35074914, ECO:0000305|PubMed:30276262, ECO:0007744|PDB:5OHS, ECO:0007744|PDB:7OFX

Chain	Residue	Details
A	GLU270
B	HIS520
C	GLU270
C	ARG283
C	LEU284
C	TRP286
C	HIS520
D	GLU270
D	ARG283
D	LEU284
D	TRP286
A	ARG283
D	HIS520
E	GLU270
E	ARG283
E	LEU284
E	TRP286
E	HIS520
F	GLU270
F	ARG283
F	LEU284
F	TRP286
A	LEU284
F	HIS520
G	GLU270
G	ARG283
G	LEU284
G	TRP286
G	HIS520
H	GLU270
H	ARG283
H	LEU284
H	TRP286
A	TRP286
H	HIS520
A	HIS520
B	GLU270
B	ARG283
B	LEU284
B	TRP286

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