5OC4

Crystal structure of human tRNA-dihydrouridine(20) synthase dsRBD R361A-R362A mutant

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	tRNA-dihydrouridine(20) synthase [NAD(P)+]-like	polymer	119	13425.3	1	UniProt (Q9NX74) Pfam (PF00035) In PDB	Homo sapiens (Human)	Dihydrouridine synthase 2,Up-regulated in lung cancer protein 8,URLC8,tRNA-dihydrouridine synthase 2-like,hDUS2
2	A	CHLORIDE ION	non-polymer		35.5	1	Chemie (CL)
3	A	GLYCEROL	non-polymer		92.1	2	Chemie (GOL)
4	A	CACODYLATE ION	non-polymer		137.0	1	Chemie (CAC)
5		water	water		18.0	144	Chemie (HOH)

Sequence modifications

A: 338 - 450 (UniProt: Q9NX74)

PDB	External Database	Details
Ala 361	Arg 361	engineered mutation
Ala 362	Arg 362	engineered mutation
His 451	-	expression tag
His 452	-	expression tag
His 453	-	expression tag
His 454	-	expression tag
His 455	-	expression tag
His 456	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	13425.3
Non-Polymers*	Number of molecules	4
	Total formula weight	356.6
All*	Total formula weight	13781.9

*Water molecules are not included.