5OC4
Crystal structure of human tRNA-dihydrouridine(20) synthase dsRBD R361A-R362A mutant
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SOLEIL BEAMLINE PROXIMA 2 |
Synchrotron site | SOLEIL |
Beamline | PROXIMA 2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2015-12-18 |
Detector | DECTRIS EIGER X 9M |
Wavelength(s) | 0.98011 |
Spacegroup name | H 3 |
Unit cell lengths | 83.940, 83.940, 56.500 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 41.970 - 1.710 |
R-factor | 0.196 |
Rwork | 0.194 |
R-free | 0.21500 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4wft |
RMSD bond length | 0.010 |
RMSD bond angle | 1.060 |
Data reduction software | XDS |
Data scaling software | STARANISO |
Phasing software | PHASER |
Refinement software | BUSTER (2.10.3) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 44.520 | 1.760 |
High resolution limit [Å] | 1.700 | 1.700 |
Rmerge | 0.217 | |
Number of reflections | 16219 | 625 |
<I/σ(I)> | 7 | 1.02 |
Completeness [%] | 91.0 | 39.01 |
Redundancy | 5.6 | 5.9 |
CC(1/2) | 0.992 | 0.260 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6 | 292 | 22% PEG 8K 100 mM sodium cacodylate |