5O4E

Crystal structure of VEGF in complex with heterodimeric Fcab JanusCT6

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Immunoglobulin gamma-1 heavy chain	polymer	223	25176.6	1	UniProt (P0DOX5) Pfam (PF07654)	Homo sapiens (Human)	Immunoglobulin gamma-1 heavy chain NIE
2	B, D (B, D)	Immunoglobulin gamma-1 heavy chain	polymer	228	25916.5	2	UniProt (P0DOX5) Pfam (PF07654)	Homo sapiens (Human)	Immunoglobulin gamma-1 heavy chain NIE
3	C (C)	Immunoglobulin gamma-1 heavy chain	polymer	222	25047.4	1	UniProt (P0DOX5) Pfam (PF07654)	Homo sapiens (Human)	Immunoglobulin gamma-1 heavy chain NIE
4	E, F (E, F)	Vascular endothelial growth factor A	polymer	96	11260.1	2	UniProt (P15692) Pfam (PF00341)	Homo sapiens (Human)	VEGF-A,Vascular permeability factor,VPF
5	G, H (G, H)	2-acetamido-2-deoxy-beta-D-glucopyranose-(1-2)-alpha-D-mannopyranose-(1-3)-[2-acetamido-2-deoxy-beta-D-glucopyranose-(1-2)-alpha-D-mannopyranose-(1-6)]beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-[alpha-L-fucopyranose-(1-6)]2-acetamido-2-deoxy-beta-D-glucopyranose	branched		1463.3	2	In PDB GlyTouCan (G80858MF)
6	I (I)	2-acetamido-2-deoxy-beta-D-glucopyranose-(1-2)-alpha-D-mannopyranose	branched		383.3	1	In PDB GlyTouCan (G85856KC)
7	J (J)	2-acetamido-2-deoxy-beta-D-glucopyranose-(1-2)-alpha-D-mannopyranose-(1-6)-[alpha-D-mannopyranose-(1-3)]beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		1114.0	1	In PDB GlyTouCan (G64481DJ)
8	K, N, O, P, Q... (A, C, D, E, F)	(4S)-2-METHYL-2,4-PENTANEDIOL	non-polymer		118.2	10	Chemie (MPD)
9	L (A)	(4R)-2-METHYLPENTANE-2,4-DIOL	non-polymer		118.2	1	Chemie (MRD)
10	M, U (A, E)	CACODYLATE ION	non-polymer		137.0	2	Chemie (CAC)
11	R (D)	2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL	non-polymer		122.1	1	Chemie (TRS)
12	AA, BA, CA, DA, Y... (C, D, E, F, A...)	water	water		18.0	242	Chemie (HOH)

Sequence modifications

A: 225 - 447 (UniProt: P0DOX5)

PDB	External Database	Details
Val 350	Thr 352	engineered mutation
Leu 366	Thr 368	engineered mutation
Leu 392	Lys 394	engineered mutation
Trp 394	Thr 396	engineered mutation

B, D: 225 - 447 (UniProt: P0DOX5)

PDB	External Database	Details
Val 350	Thr 352	engineered mutation
Tyr 351	Leu 353	engineered mutation
Arg 359	Thr 361	engineered mutation
Phe 360	Lys 362	engineered mutation
Tyr 361	Asn 363	engineered mutation
Asp 388	-	insertion
Ile 389	-	insertion
Phe 389	-	insertion
Pro 389	-	insertion
Asn 389	-	insertion
Gly 389	Glu 390	engineered mutation
Leu 389	Asn 391	engineered mutation
Ala 405	Phe 407	engineered mutation
Val 407	Tyr 409	engineered mutation
Pro 413	Asp 415	engineered mutation
Tyr 414	Lys 416	engineered mutation
Pro 415	Ser 417	engineered mutation
Ser 416	Arg 418	engineered mutation
Leu 418	Gln 420	engineered mutation
Met 419	Gln 421	engineered mutation
Thr 421	Asn 423	engineered mutation
Arg 422	Val 424	engineered mutation
His 440	Ser 442	engineered mutation
Glu 442	Ser 444	engineered mutation
Tyr 443	Leu 445	engineered mutation
Gln 444	Ser 446	engineered mutation
Trp 445	Pro 447	engineered mutation
Pro 446	Gly 448	engineered mutation
Thr 447	Lys 449	engineered mutation

C: 225 - 446 (UniProt: P0DOX5)

PDB	External Database	Details
Val 350	Thr 352	engineered mutation
Leu 366	Thr 368	engineered mutation
Leu 392	Lys 394	engineered mutation
Trp 394	Thr 396	engineered mutation

E, F: 13 - 108 (UniProt: P15692)

PDB	External Database	Details
Met 13	Glu 39	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	6
	Total formula weight	124577.2
Branched	Number of molecules	4
	Total formula weight	4424.1
Non-Polymers*	Number of molecules	14
	Total formula weight	1696.0
All*	Total formula weight	130697.3

*Water molecules are not included.