5O4E
Crystal structure of VEGF in complex with heterodimeric Fcab JanusCT6
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE MASSIF-1 |
| Synchrotron site | ESRF |
| Beamline | MASSIF-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-09-01 |
| Detector | DECTRIS PILATUS3 2M |
| Wavelength(s) | 0.9677 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 89.499, 130.345, 139.168 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 44.750 - 2.150 |
| R-factor | 0.2007 |
| Rwork | 0.200 |
| R-free | 0.23630 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.610 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | BALBES |
| Refinement software | PHENIX ((dev_2747: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.750 | 2.227 |
| High resolution limit [Å] | 2.150 | 2.150 |
| Rmerge | 0.107 | 1.800 |
| Rpim | 0.054 | 1.104 |
| Number of reflections | 87083 | 7766 |
| <I/σ(I)> | 8.75 | |
| Completeness [%] | 97.8 | 88.52 |
| Redundancy | 4.5 | 3.1 |
| CC(1/2) | 0.997 | 0.198 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 295 | 0.1 M Sodium cacodylate, 40% (v/v) MPD, 5% (w/v) PEG 8000 |
