5NX2

Crystal structure of thermostabilised full-length GLP-1R in complex with a truncated peptide agonist at 3.7 A resolution

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	Glucagon-like peptide 1 receptor	polymer	422	49028.7	1	UniProt (P43220) Pfam (PF02793) Pfam (PF00002) In PDB	Homo sapiens (Human)	GLP-1R
2	B	truncated peptide agonist	polymer	10	1488.6	1		synthetic construct
3	C	2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		424.4	1	In PDB GlyTouCan (G42666HT)
4	A	2-acetamido-2-deoxy-beta-D-glucopyranose	non-polymer		221.2	1	Chemie (NAG)
5	A	octyl 1-thio-beta-D-glucopyranoside	non-polymer		308.4	3	Chemie (SOG)

Sequence modifications

A: 24 - 432 (UniProt: P43220)

PDB	External Database	Details
Gly 20	-	expression tag
Pro 21	-	expression tag
Ala 22	-	expression tag
Ser 23	-	expression tag
Glu 207	Thr 207	engineered mutation
Ala 211	Gln 211	engineered mutation
Arg 215	Asp 215	engineered mutation
Phe 232	Leu 232	engineered mutation
Phe 260	Leu 260	variant
Ala 295	Gly 295	engineered mutation
Ala 298	Thr 298	engineered mutation
Ala 329	Cys 329	engineered mutation
Ala 358	Pro 358	engineered mutation
Ala 361	Gly 361	engineered mutation
Val 363	His 363	engineered mutation
Ala 405	Val 405	engineered mutation
Ala 433	-	expression tag
Ala 434	-	expression tag
Ala 435	-	expression tag
Leu 436	-	expression tag
Glu 437	-	expression tag
Val 438	-	expression tag
Leu 439	-	expression tag
Phe 440	-	expression tag
Gln 441	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	50517.3
Branched	Number of molecules	1
	Total formula weight	424.4
Non-Polymers*	Number of molecules	4
	Total formula weight	1146.5
All*	Total formula weight	52088.2

*Water molecules are not included.