5NT2

Complex of influenza A NS1 with TRIM25 coiled coil domain

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	F, C, D, E	Non-structural protein 1	polymer	233	25867.6	4	UniProt (P03496) Pfam (PF00600) In PDB	Influenza A virus (strain A/Puerto Rico/8/1934 H1N1)	NS1,NS1A
2	I, A, V, N	E3 ubiquitin/ISG15 ligase TRIM25	polymer	193	22011.9	4	UniProt (Q14258) In PDB	Homo sapiens (Human)	Estrogen-responsive finger protein,RING finger protein 147,RING-type E3 ubiquitin transferase,RING-type E3 ubiquitin transferase TRIM25,Tripartite motif-containing protein 25,Ubiquitin/ISG15-conjugating enzyme TRIM25,Zinc finger protein 147

Sequence modifications

F, C, D, E: 1 - 230 (UniProt: P03496)

PDB	External Database	Details
Gly -2	-	expression tag
Pro -1	-	expression tag
Gly 0	-	expression tag
Ala 38	Arg 38	engineered mutation
Ala 41	Lys 41	engineered mutation
Glu 101	Asp 101	variant
Ala 187	Trp 187	engineered mutation

I, A, V, N: 190 - 379 (UniProt: Q14258)

PDB	External Database	Details
Gly 187	-	expression tag
Pro 188	-	expression tag
Gly 189	-	expression tag
Leu 358	Pro 358	variant

Contents of the asymmetric unit

Polymers	Number of chains	8
	Total formula weight	191518.3
All*	Total formula weight	191518.3

*Water molecules are not included.