5NT2

Complex of influenza A NS1 with TRIM25 coiled coil domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04
Synchrotron site	Diamond
Beamline	I04
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-07-10
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.9795
Spacegroup name	P 1
Unit cell lengths	73.257, 76.296, 92.072
Unit cell angles	100.04, 93.71, 111.14

Refinement procedure

Resolution	61.684 - 4.259
R-factor	0.2721
Rwork	0.270
R-free	0.30880
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4ltb
RMSD bond length	0.004
RMSD bond angle	0.800
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((1.12_2829: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	61.510	4.330
High resolution limit [Å]	4.259	4.260
Rmerge	0.190
Rpim	0.140
Number of reflections	12516	630
<I/σ(I)>	5.7	2.5
Completeness [%]	98.6	98.6
Redundancy	2.6
CC(1/2)	0.985	0.800

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	291	100 mM sodium citrate pH 6.5, 24% PEG 3350