5N9G

TFIIIB -TBP/Brf2/DNA and SANT domain of Bdp1-

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, F	Transcription factor IIIB 50 kDa subunit	polymer	377	42195.6	2	UniProt (Q9HAW0) Pfam (PF21886) In PDB	Homo sapiens (Human)	hTFIIIB50,B-related factor 2,BRF-2,hBRFU
2	B, G	TATA-box-binding protein	polymer	200	22589.7	2	UniProt (P20226) Pfam (PF00352) In PDB	Homo sapiens (Human)	TATA sequence-binding protein,TATA-binding factor,TATA-box factor,Transcription initiation factor TFIID TBP subunit
3	C, H	Transcription factor TFIIIB component B'' homolog	polymer	175	20414.3	2	UniProt (A6H8Y1) Pfam (PF15963) In PDB	Homo sapiens (Human)	Transcription factor IIIB 150,TFIIIB150,Transcription factor-like nuclear regulator
4	D, I	DNA/RNA (27-MER)	polymer	27	8363.3	2		Homo sapiens
5	E, J	DNA/RNA (25-MER)	polymer	25	8026.1	2		Homo sapiens
6	H	SODIUM ION	non-polymer		23.0	1	Chemie (NA)
7		water	water		18.0	298	Chemie (HOH)

Sequence modifications

A, F: 62 - 419 (UniProt: Q9HAW0)

PDB	External Database	Details
Met 43	-	initiating methionine
Ala 44	-	expression tag
His 45	-	expression tag
His 46	-	expression tag
His 47	-	expression tag
His 48	-	expression tag
His 49	-	expression tag
His 50	-	expression tag
Ser 51	-	expression tag
Ser 52	-	expression tag
Gly 53	-	expression tag
Leu 54	-	expression tag
Glu 55	-	expression tag
Val 56	-	expression tag
Leu 57	-	expression tag
Phe 58	-	expression tag
Gln 59	-	expression tag
Gly 60	-	expression tag
Pro 61	-	expression tag

B, G: 159 - 339 (UniProt: P20226)

PDB	External Database	Details
Met 140	-	initiating methionine
Ala 141	-	expression tag
His 142	-	expression tag
His 143	-	expression tag
His 144	-	expression tag
His 145	-	expression tag
His 146	-	expression tag
His 147	-	expression tag
Ser 148	-	expression tag
Ser 149	-	expression tag
Gly 150	-	expression tag
Leu 151	-	expression tag
Glu 152	-	expression tag
Val 153	-	expression tag
Leu 154	-	expression tag
Phe 155	-	expression tag
Gln 156	-	expression tag
Gly 157	-	expression tag
Pro 158	-	expression tag

C, H: 241 - 396 (UniProt: A6H8Y1)

PDB	External Database	Details
Met 222	-	initiating methionine
Ala 223	-	expression tag
His 224	-	expression tag
His 225	-	expression tag
His 226	-	expression tag
His 227	-	expression tag
His 228	-	expression tag
His 229	-	expression tag
Ser 230	-	expression tag
Ser 231	-	expression tag
Gly 232	-	expression tag
Leu 233	-	expression tag
Glu 234	-	expression tag
Val 235	-	expression tag
Leu 236	-	expression tag
Phe 237	-	expression tag
Gln 238	-	expression tag
Gly 239	-	expression tag
Pro 240	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	10
	Total formula weight	203177.9
Non-Polymers*	Number of molecules	1
	Total formula weight	23.0
All*	Total formula weight	203200.9

*Water molecules are not included.