5N9G

TFIIIB -TBP/Brf2/DNA and SANT domain of Bdp1-

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID29
Synchrotron site	ESRF
Beamline	ID29
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-02-17
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.919760
Spacegroup name	P 1 21 1
Unit cell lengths	81.190, 124.080, 88.610
Unit cell angles	90.00, 99.51, 90.00

Refinement procedure

Resolution	49.040 - 2.700
R-factor	0.196
Rwork	0.194
R-free	0.23400
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4roc
RMSD bond length	0.010
RMSD bond angle	1.030
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	BUSTER (2.10.3)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.040	2.790
High resolution limit [Å]	2.700	2.700
Rpim	0.161	1.552
Number of reflections	47164
<I/σ(I)>	5.3	0.7
Completeness [%]	99.2	99.7
Redundancy	9.2	9.3
CC(1/2)	0.988	0.257

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	10-16% PEG 8000, 150-200 mM KCl, 100 mM MES pH 6.5, 10-15 mM MgCl2, 1 mM TCEP