5N4E

Prolyl oligopeptidase B from Galerina marginata bound to 35mer hydrolysis and macrocyclization substrate - H698A mutant

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	Prolyl oligopeptidase	polymer	765	85641.8	2	UniProt (H2E7Q8) Pfam (PF02897) Pfam (PF00326)	Galerina marginata
2	C, D (C, D)	Alpha-amanitin proprotein	polymer	35	3850.3	2	UniProt (H2E7Q5) UniProt (by SIFTS) (A0A067SLB9)	Galerina marginata
3	E (B)	GLYCEROL	non-polymer		92.1	1	Chemie (GOL)
4	F, G, H, I (A, B, C, D)	water	water		18.0	939	Chemie (HOH)

Sequence modifications

A, B: 1 - 730 (UniProt: H2E7Q8)

PDB	External Database	Details
Ala 698	His 698	engineered mutation
Met 731	-	expression tag
Phe 732	-	expression tag
Asp 733	-	expression tag
Thr 734	-	expression tag
Asn 735	-	expression tag
Ala 736	-	expression tag
Thr 737	-	expression tag
Arg 738	-	expression tag
Leu 739	-	expression tag
Pro 740	-	expression tag
Ile 741	-	expression tag
Trp 742	-	expression tag
Gly 743	-	expression tag
Ile 744	-	expression tag
Gly 745	-	expression tag
Cys 746	-	expression tag
Asn 747	-	expression tag
Pro 748	-	expression tag
Trp 749	-	expression tag
Thr 750	-	expression tag
Ala 751	-	expression tag
Glu 752	-	expression tag
His 753	-	expression tag
Val 754	-	expression tag
Asp 755	-	expression tag
Gln 756	-	expression tag
Thr 757	-	expression tag
Leu 758	-	expression tag
Ala 759	-	expression tag
Ser 760	-	expression tag
Gly 761	-	expression tag
Asn 762	-	expression tag
Asp 763	-	expression tag
Ile 764	-	expression tag
Cys 765	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	178984.1
Non-Polymers*	Number of molecules	1
	Total formula weight	92.1
All*	Total formula weight	179076.2

*Water molecules are not included.