5N48

Structure of Anticalin N9B in complex with extra-domain B of human oncofetal fibronectin

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, C (A, C)	Neutrophil gelatinase-associated lipocalin	polymer	188	21659.8	2	UniProt (P80188) Pfam (PF00061)	Homo sapiens (Human)	NGAL,25 kDa alpha-2-microglobulin-related subunit of MMP-9,Lipocalin-2,Oncogene 24p3,Siderocalin LCN2,p25
2	B, D (B, D)	Fibronectin	polymer	106	11334.5	2	UniProt (P02751) Pfam (PF00041)	Homo sapiens (Human)	FN,Cold-insoluble globulin,CIG
3	E, F, G, H (A, B, C, D)	water	water		18.0	296	Chemie (HOH)

Sequence modifications

A, C: 1 - 178 (UniProt: P80188)

PDB	External Database	Details
His 28	Gln 48	engineered mutation
Arg 36	Leu 56	engineered mutation
Met 40	Ala 60	engineered mutation
Arg 41	Ile 61	engineered mutation
Ala 49	Gln 69	engineered mutation
Val 52	Tyr 72	engineered mutation
Lys 68	Ser 88	engineered mutation
Met 70	Leu 90	engineered mutation
Gln 72	Arg 92	engineered mutation
Arg 73	Lys 93	engineered mutation
Lys 77	Asp 97	engineered mutation
Met 79	Trp 99	engineered mutation
Asn 81	Arg 101	engineered mutation
Ser 87	Cys 107	engineered mutation
Ala 96	Asn 116	engineered mutation
Pro 100	Tyr 120	engineered mutation
Pro 103	Leu 123	engineered mutation
Thr 106	Tyr 126	engineered mutation
His 125	Lys 145	engineered mutation
Phe 127	Ser 147	engineered mutation
His 134	Lys 154	engineered mutation
Ser 179	-	expression tag
Ala 180	-	expression tag
Trp 181	-	expression tag
Ser 182	-	expression tag
His 183	-	expression tag
Pro 184	-	expression tag
Gln 185	-	expression tag
Phe 186	-	expression tag
Glu 187	-	expression tag
Lys 188	-	expression tag

B, D: 1266 - 1357 (UniProt: P02751)

PDB	External Database	Details
Met 1252	-	initiating methionine
Ala 1253	-	expression tag
Ser 1254	-	expression tag
Arg 1255	-	expression tag
Gly 1256	-	expression tag
Ser 1257	-	expression tag
His 1258	-	expression tag
His 1259	-	expression tag
His 1260	-	expression tag
His 1261	-	expression tag
His 1262	-	expression tag
His 1263	-	expression tag
Gly 1264	-	expression tag
Ala 1265	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	65988.5
All*	Total formula weight	65988.5

*Water molecules are not included.