5N2H
Structure of the E9 DNA polymerase exonuclease deficient mutant (D166A+E168A) from vaccinia virus
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | DNA polymerase | polymer | 1009 | 117214.2 | 1 | UniProt (P20509) Pfam (PF08452) Pfam (PF03104) Pfam (PF08408) Pfam (PF00136) | Vaccinia virus Copenhagen | Vaccinia virus E9 DNA polymerase exo- mutant |
| 2 | B, C, D (A) | 2-(N-MORPHOLINO)-ETHANESULFONIC ACID | non-polymer | 195.2 | 3 | Chemie (MES) | |||
| 3 | E, F (A) | GLYCEROL | non-polymer | 92.1 | 2 | Chemie (GOL) | |||
| 4 | G (A) | 2,3-DIHYDROXY-1,4-DITHIOBUTANE | non-polymer | 154.3 | 1 | Chemie (DTT) | |||
| 5 | H (A) | 4-(2-HYDROXYETHYL)-1-PIPERAZINE ETHANESULFONIC ACID | non-polymer | 238.3 | 1 | Chemie (EPE) | |||
| 6 | I (A) | water | water | 18.0 | 214 | Chemie (HOH) |
Sequence modifications
A: 2 - 1005 (UniProt: P20509)
| PDB | External Database | Details |
|---|---|---|
| Gly -3 | - | expression tag |
| Ala -2 | - | expression tag |
| Met -1 | - | expression tag |
| Asp 0 | - | expression tag |
| Pro 1 | - | expression tag |
| Ala 166 | Asp 166 | engineered mutation |
| Ala 168 | Glu 168 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 1 |
| Total formula weight | 117214.2 | |
| Non-Polymers* | Number of molecules | 7 |
| Total formula weight | 1162.5 | |
| All* | Total formula weight | 118376.7 |
