5N2H
Structure of the E9 DNA polymerase exonuclease deficient mutant (D166A+E168A) from vaccinia virus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-07-17 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 1.2724 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 133.524, 133.524, 229.492 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 57.000 - 2.810 |
| R-factor | 0.18662 |
| Rwork | 0.185 |
| R-free | 0.22165 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5n2g |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.617 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 57.000 | 2.960 |
| High resolution limit [Å] | 2.810 | 2.810 |
| Rmeas | 0.089 | 0.788 |
| Number of reflections | 57789 | |
| <I/σ(I)> | 11.3 | 1.8 |
| Completeness [%] | 99.0 | 97.1 |
| Redundancy | 3.3 | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.25 | 293 | 9-11% PEG 3000, 20-25% glycerol, 100 mM MES-NaOH pH 6.25 |
