5MZQ
X-ray structure of the M205W mutant of GLIC in complex with bromoform
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B, C, D, E (A, B, C, D, E) | Proton-gated ion channel | polymer | 327 | 37473.9 | 5 | UniProt (Q7NDN8) Pfam (PF02931) | Gloeobacter violaceus (strain PCC 7421) | GLIC,Ligand-gated ion channel,LGIC |
| 2 | AA, F, GA, KA, N... (C, A, D, B, E) | ACETATE ION | non-polymer | 59.0 | 10 | Chemie (ACT) | |||
| 3 | BA, G, LA, R, UA (C, A, D, B, E) | 1,2-DIOCTANOYL-SN-GLYCERO-3-PHOSPHOCHOLINE | non-polymer | 510.6 | 5 | Chemie (PC8) | |||
| 4 | CA, H, MA, S, VA (C, A, D, B, E) | (3R)-3-(dodecanoyloxy)tetradecanoic acid | non-polymer | 426.7 | 5 | Chemie (2IL) | |||
| 5 | AB, DA, HA, I, NA... (E, C, A, D, B) | DODECANE | non-polymer | 170.3 | 11 | Chemie (D12) | |||
| 6 | EA, J, K, L, OA... (C, A, D, B, E) | CHLORIDE ION | non-polymer | 35.5 | 7 | Chemie (CL) | |||
| 7 | FA, JA, M, PA, V... (C, D, A, B, E) | SODIUM ION | non-polymer | 23.0 | 6 | Chemie (NA) | |||
| 8 | BB, IA, P, SA, Y (E, C, A, D, B) | TRIBROMOMETHANE | non-polymer | 252.7 | 5 | Chemie (MBR) | |||
| 9 | CB, DB, EB, FB, GB (A, B, C, D, E) | water | water | 18.0 | 400 | Chemie (HOH) |
Sequence modifications
A, B, C, D, E: 1 - 317 (UniProt: Q7NDN8)
| PDB | External Database | Details |
|---|---|---|
| Trp 205 | Met 247 | engineered mutation |
| Gly 318 | - | expression tag |
| Tyr 319 | - | expression tag |
| Pro 320 | - | expression tag |
| Tyr 321 | - | expression tag |
| Asp 322 | - | expression tag |
| Val 323 | - | expression tag |
| Pro 324 | - | expression tag |
| Asp 325 | - | expression tag |
| Tyr 326 | - | expression tag |
| Ala 327 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 5 |
| Total formula weight | 187369.6 | |
| Non-Polymers* | Number of molecules | 49 |
| Total formula weight | 8800.4 | |
| All* | Total formula weight | 196169.9 |
