5MQJ

Crystal structure of dCK mutant C3S

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D (A, B, C, D)	Deoxycytidine kinase	polymer	281	33040.1	4	UniProt (P27707) Pfam (PF01712)	Homo sapiens (Human)	dCK
2	E, H, K, N (A, B, C, D)	URIDINE-5'-DIPHOSPHATE	non-polymer		404.2	4	Chemie (UDP)
3	F, I, L, O (A, B, C, D)	2'-DEOXYURIDINE 5'-MONOPHOSPHATE	non-polymer		308.2	4	Chemie (UMP)
4	G, J, M, P (A, B, C, D)	MAGNESIUM ION	non-polymer		24.3	4	Chemie (MG)

Sequence modifications

A, B, C, D: 2 - 260 (UniProt: P27707)

PDB	External Database	Details
Met -20	-	initiating methionine
Ser -19	-	expression tag
Tyr -18	-	expression tag
Tyr -17	-	expression tag
His -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
Leu -10	-	expression tag
Glu -9	-	expression tag
Ser -8	-	expression tag
Thr -7	-	expression tag
Ser -6	-	expression tag
Leu -5	-	expression tag
Tyr -4	-	expression tag
Lys -3	-	expression tag
Lys -2	-	expression tag
Ala -1	-	expression tag
Gly 0	-	expression tag
Leu 1	-	expression tag
Ser 9	Cys 9	engineered mutation
Ser 45	Cys 45	engineered mutation
Ser 59	Cys 59	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	132160.4
Non-Polymers*	Number of molecules	12
	Total formula weight	2946.6
All*	Total formula weight	135107.0

*Water molecules are not included.