5MLM

Plantago Major multifunctional oxidoreductase V150M mutant in complex with progesterone and NADP+

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	Progesterone 5-beta-reductase	polymer	411	46820.8	1	UniProt (D6N9X1) Pfam (PF01370) In PDB	Plantago major
2	A	PROGESTERONE	non-polymer		314.5	1	Chemie (STR)
3	A	NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE	non-polymer		743.4	1	Chemie (NAP)
4		water	water		18.0	60	Chemie (HOH)

Sequence modifications

A: 1 - 389 (UniProt: D6N9X1)

PDB	External Database	Details
Met -21	-	initiating methionine
Gly -20	-	expression tag
His -19	-	expression tag
His -18	-	expression tag
His -17	-	expression tag
His -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
Asp -13	-	expression tag
Tyr -12	-	expression tag
Asp -11	-	expression tag
Ile -10	-	expression tag
Pro -9	-	expression tag
Thr -8	-	expression tag
Thr -7	-	expression tag
Glu -6	-	expression tag
Asn -5	-	expression tag
Leu -4	-	expression tag
Tyr -3	-	expression tag
Phe -2	-	expression tag
Gln -1	-	expression tag
Gly 0	-	expression tag
Met 150	Val 150	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	46820.8
Non-Polymers*	Number of molecules	2
	Total formula weight	1057.9
All*	Total formula weight	47878.7

*Water molecules are not included.