5MI8

Structure of the phosphomimetic mutant of EF-Tu T383E

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	Elongation factor Tu 1	polymer	402	44357.5	2	UniProt (A8A5E6) Pfam (PF00009) Pfam (PF03144) Pfam (PF03143) UniProt (by SIFTS) (P0CE47)	Escherichia coli HS	EF-Tu 1
2	C, D, L, M (A, B)	MAGNESIUM ION	non-polymer		24.3	4	Chemie (MG) PubChem (888)
3	E, N (A, B)	GUANOSINE-5'-DIPHOSPHATE	non-polymer		443.2	2	Chemie (GDP) PubChem (136073927) PubChem (135398619) PubChem (135402030) PubChem (135431654) PubChem (135566337) PubChem (135760556) PubChem (136026707) PubChem (136053418) PubChem (136663964) PubChem (136664748) PubChem (136759848) PubChem (136759849) PubChem (136759850) PubChem (136836636) PubChem (136847074) PubChem (172898537)
4	F, G, H, O (A, B)	ACETATE ION	non-polymer		59.0	4	Chemie (ACT) PubChem (175)
5	I, P (A, B)	4-(2-HYDROXYETHYL)-1-PIPERAZINE ETHANESULFONIC ACID	non-polymer		238.3	2	Chemie (EPE) PubChem (23831)
6	J, K (A)	BETA-MERCAPTOETHANOL	non-polymer		78.1	2	Chemie (BME)
7	Q (B)	CHLORIDE ION	non-polymer		35.5	1	Chemie (CL) PubChem (312)
8	R, S (A, B)	water	water		18.0	201	Chemie (HOH)

Sequence modifications

A, B: 2 - 394 (UniProt: A8A5E6)

PDB	External Database	Details
Met -7	-	initiating methionine
Gly -6	-	expression tag
Ser -5	-	expression tag
His -4	-	expression tag
His -3	-	expression tag
His -2	-	expression tag
His -1	-	expression tag
His 0	-	expression tag
His 1	-	expression tag
Cys 222	Ser 222	engineered mutation
Glu 383	Thr 383	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	88715.1
Non-Polymers*	Number of molecules	15
	Total formula weight	1888.1
All*	Total formula weight	90603.2

*Water molecules are not included.