5MI8
Structure of the phosphomimetic mutant of EF-Tu T383E
Entity
Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
1 | A, B | Elongation factor Tu 1 | polymer | 402 | 44357.5 | 2 | UniProt (A8A5E6) Pfam (PF00009) Pfam (PF03144) Pfam (PF03143) UniProt (by SIFTS) (P0CE47) In PDB | Escherichia coli HS | EF-Tu 1 |
2 | A, B | MAGNESIUM ION | non-polymer | 24.3 | 4 | Chemie (MG) | |||
3 | A, B | GUANOSINE-5'-DIPHOSPHATE | non-polymer | 443.2 | 2 | Chemie (GDP) | |||
4 | A, B | ACETATE ION | non-polymer | 59.0 | 4 | Chemie (ACT) | |||
5 | A, B | 4-(2-HYDROXYETHYL)-1-PIPERAZINE ETHANESULFONIC ACID | non-polymer | 238.3 | 2 | Chemie (EPE) | |||
6 | A | BETA-MERCAPTOETHANOL | non-polymer | 78.1 | 2 | Chemie (BME) | |||
7 | B | CHLORIDE ION | non-polymer | 35.5 | 1 | Chemie (CL) | |||
8 | water | water | 18.0 | 201 | Chemie (HOH) |
Sequence modifications
A, B: 2 - 394 (UniProt: A8A5E6)
PDB | External Database | Details |
---|---|---|
Met -7 | - | initiating methionine |
Gly -6 | - | expression tag |
Ser -5 | - | expression tag |
His -4 | - | expression tag |
His -3 | - | expression tag |
His -2 | - | expression tag |
His -1 | - | expression tag |
His 0 | - | expression tag |
His 1 | - | expression tag |
Cys 222 | Ser 222 | engineered mutation |
Glu 383 | Thr 383 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
Polymers | Number of chains | 2 |
Total formula weight | 88715.1 | |
Non-Polymers* | Number of molecules | 15 |
Total formula weight | 1888.1 | |
All* | Total formula weight | 90603.2 |