5MI8

Structure of the phosphomimetic mutant of EF-Tu T383E

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	Elongation factor Tu 1	polymer	402	44357.5	2	UniProt (A8A5E6) Pfam (PF00009) Pfam (PF03144) Pfam (PF03143) UniProt (by SIFTS) (P0CE47)	Escherichia coli HS	EF-Tu 1
2	C, D, L, M (A, B)	MAGNESIUM ION	non-polymer		24.3	4	Chemie (MG)
3	E, N (A, B)	GUANOSINE-5'-DIPHOSPHATE	non-polymer		443.2	2	Chemie (GDP)
4	F, G, H, O (A, B)	ACETATE ION	non-polymer		59.0	4	Chemie (ACT)
5	I, P (A, B)	4-(2-HYDROXYETHYL)-1-PIPERAZINE ETHANESULFONIC ACID	non-polymer		238.3	2	Chemie (EPE)
6	J, K (A)	BETA-MERCAPTOETHANOL	non-polymer		78.1	2	Chemie (BME)
7	Q (B)	CHLORIDE ION	non-polymer		35.5	1	Chemie (CL)
8	R, S (A, B)	water	water		18.0	201	Chemie (HOH)

Sequence modifications

A, B: 2 - 394 (UniProt: A8A5E6)

PDB	External Database	Details
Met -7	-	initiating methionine
Gly -6	-	expression tag
Ser -5	-	expression tag
His -4	-	expression tag
His -3	-	expression tag
His -2	-	expression tag
His -1	-	expression tag
His 0	-	expression tag
His 1	-	expression tag
Cys 222	Ser 222	engineered mutation
Glu 383	Thr 383	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	88715.1
Non-Polymers*	Number of molecules	15
	Total formula weight	1888.1
All*	Total formula weight	90603.2

*Water molecules are not included.