5MI8
Structure of the phosphomimetic mutant of EF-Tu T383E
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I24 |
| Synchrotron site | Diamond |
| Beamline | I24 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-12-04 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.8696 |
| Spacegroup name | P 1 |
| Unit cell lengths | 55.870, 61.510, 65.040 |
| Unit cell angles | 71.53, 71.17, 87.76 |
Refinement procedure
| Resolution | 52.750 - 2.180 |
| R-factor | 0.189 |
| Rwork | 0.187 |
| R-free | 0.22490 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.270 |
| Data reduction software | xia2 |
| Data scaling software | xia2 |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.10.2) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 52.800 |
| High resolution limit [Å] | 2.180 |
| Rmeas | 0.105 |
| Number of reflections | 38398 |
| <I/σ(I)> | 8.3 |
| Completeness [%] | 90.0 |
| Redundancy | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 0.1 M MgCl2, 0.1 M HEPES and 10 % PEG4000 |
