5MFV

Crystal structure of the GluK1 ligand-binding domain in complex with kainate and BPAM-521 at 2.18 A resolution

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	Glutamate receptor ionotropic, kainate 1,Glutamate receptor ionotropic, kainate 1	polymer	257	29108.5	2	UniProt (P22756) Pfam (PF10613) Pfam (PF00060)	Rattus norvegicus (Rat)	GluK1,Glutamate receptor 5,GluR5,GluK1,Glutamate receptor 5,GluR5
2	C, D (A)	4-Cyclopropyl-3,4-dihydro-7-hydroxy-2H-1,2,4-benzothiadiazine 1,1-dioxide	non-polymer		240.3	2	Chemie (5PX) PubChem (67305878)
3	E, I (A, B)	3-(CARBOXYMETHYL)-4-ISOPROPENYLPROLINE	non-polymer		213.2	2	Chemie (KAI) PubChem (3816) PubChem (23616889) PubChem (10255) PubChem (73345) PubChem (45039619) PubChem (3083828) PubChem (5317452) PubChem (5318769) PubChem (6603893) PubChem (6453379) PubChem (6604705) PubChem (101994997) PubChem (10036135) PubChem (11095940) PubChem (135037246)
4	F (A)	CHLORIDE ION	non-polymer		35.5	1	Chemie (CL) PubChem (312)
5	G, J, K (A, B)	SULFATE ION	non-polymer		96.1	3	Chemie (SO4)
6	H (A)	GLYCEROL	non-polymer		92.1	1	Chemie (GOL) PubChem (753)
7	L (B)	ACETATE ION	non-polymer		59.0	1	Chemie (ACT) PubChem (175)
8	M, N (A, B)	water	water		18.0	324	Chemie (HOH)

Sequence modifications

A, B: 430 - 544 (UniProt: P22756)

PDB	External Database	Details
Gly 429	-	cloning artifact
Gly 462	Ala 477	see remark 999

A, B: 667 - 805 (UniProt: P22756)

PDB	External Database	Details
Gly 545	-	linker
Thr 546	-	linker

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	58216.9
Non-Polymers*	Number of molecules	10
	Total formula weight	1381.8
All*	Total formula weight	59598.7

*Water molecules are not included.