5MFS

The crystal structure of E. coli Aminopeptidase N in complex with 7-amino-4-phenyl-5,7,8,9-tetrahydrobenzocyclohepten-6-one

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Aminopeptidase N	polymer	891	101441.2	1	UniProt (P04825) Pfam (PF17900) Pfam (PF01433) Pfam (PF11940) Pfam (PF17432)	Escherichia coli	Alpha-aminoacylpeptide hydrolase
2	B (A)	ZINC ION	non-polymer		65.4	1	Chemie (ZN)
3	C (A)	[(7~{S})-6,6-bis(oxidanyl)-4-phenyl-5,7,8,9-tetrahydrobenzo[7]annulen-7-yl]azanium	non-polymer		270.3	1	Chemie (7ML)
4	D, E, F, G, H... (A)	SODIUM ION	non-polymer		23.0	9	Chemie (NA)
5	M, N, O, P, Q (A)	MALONATE ION	non-polymer		102.0	5	Chemie (MLI)
6	R, S, T, U, V (A)	DIMETHYL SULFOXIDE	non-polymer		78.1	5	Chemie (DMS)
7	W, X, Y, Z (A)	CHLORIDE ION	non-polymer		35.5	4	Chemie (CL)
8	AA, BA, CA, DA, EA... (A)	GLYCEROL	non-polymer		92.1	14	Chemie (GOL)
9	OA (A)	water	water		18.0	1763	Chemie (HOH)

Sequence modifications

A: 1 - 870 (UniProt: P04825)

PDB	External Database	Details
Met -20	-	initiating methionine
Gly -19	-	expression tag
Ser -18	-	expression tag
Ser -17	-	expression tag
His -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
Ser -10	-	expression tag
Ser -9	-	expression tag
Gly -8	-	expression tag
Glu -7	-	expression tag
Asn -6	-	expression tag
Leu -5	-	expression tag
Tyr -4	-	expression tag
Phe -3	-	expression tag
Gln -2	-	expression tag
Gly -1	-	expression tag
His 0	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	101441.2
Non-Polymers*	Number of molecules	39
	Total formula weight	2874.7
All*	Total formula weight	104315.9

*Water molecules are not included.