5MFS
The crystal structure of E. coli Aminopeptidase N in complex with 7-amino-4-phenyl-5,7,8,9-tetrahydrobenzocyclohepten-6-one
Functional Information from GO Data
| Chain | GOid | namespace | contents |
| A | 0004177 | molecular_function | aminopeptidase activity |
| A | 0005515 | molecular_function | protein binding |
| A | 0005886 | cellular_component | plasma membrane |
| A | 0006508 | biological_process | proteolysis |
| A | 0008233 | molecular_function | peptidase activity |
| A | 0008237 | molecular_function | metallopeptidase activity |
| A | 0008270 | molecular_function | zinc ion binding |
| A | 0016285 | molecular_function | alanyl aminopeptidase activity |
| A | 0016787 | molecular_function | hydrolase activity |
| A | 0042802 | molecular_function | identical protein binding |
| A | 0046872 | molecular_function | metal ion binding |
Functional Information from PDB Data
| site_id | AC1 |
| Number of Residues | 4 |
| Details | binding site for residue ZN A 1001 |
| Chain | Residue |
| A | HIS297 |
| A | HIS301 |
| A | GLU320 |
| A | 7ML1002 |
| site_id | AC2 |
| Number of Residues | 13 |
| Details | binding site for residue 7ML A 1002 |
| Chain | Residue |
| A | HIS297 |
| A | GLU298 |
| A | HIS301 |
| A | LYS319 |
| A | GLU320 |
| A | TYR381 |
| A | ZN1001 |
| A | MLI1012 |
| A | GLU121 |
| A | MET260 |
| A | ALA262 |
| A | GLU264 |
| A | ARG293 |
| site_id | AC3 |
| Number of Residues | 6 |
| Details | binding site for residue NA A 1003 |
| Chain | Residue |
| A | SER332 |
| A | ASP333 |
| A | GLY335 |
| A | HOH1927 |
| A | HOH2214 |
| A | HOH2302 |
| site_id | AC4 |
| Number of Residues | 6 |
| Details | binding site for residue NA A 1004 |
| Chain | Residue |
| A | HIS771 |
| A | PHE774 |
| A | HOH1322 |
| A | HOH1559 |
| A | HOH2247 |
| A | HOH2291 |
| site_id | AC5 |
| Number of Residues | 6 |
| Details | binding site for residue NA A 1005 |
| Chain | Residue |
| A | SER508 |
| A | HOH1882 |
| A | HOH2060 |
| A | HOH2145 |
| A | HOH2340 |
| A | HOH2806 |
| site_id | AC6 |
| Number of Residues | 7 |
| Details | binding site for residue NA A 1006 |
| Chain | Residue |
| A | GLU123 |
| A | GLU371 |
| A | HOH1145 |
| A | HOH1497 |
| A | HOH1675 |
| A | HOH1872 |
| A | HOH2314 |
| site_id | AC7 |
| Number of Residues | 6 |
| Details | binding site for residue NA A 1007 |
| Chain | Residue |
| A | ASP452 |
| A | TYR544 |
| A | HOH1555 |
| A | HOH2295 |
| A | HOH2330 |
| A | HOH2508 |
| site_id | AC8 |
| Number of Residues | 6 |
| Details | binding site for residue NA A 1008 |
| Chain | Residue |
| A | GLU371 |
| A | HOH1154 |
| A | HOH1327 |
| A | HOH1605 |
| A | HOH2314 |
| A | HOH2481 |
| site_id | AC9 |
| Number of Residues | 6 |
| Details | binding site for residue NA A 1009 |
| Chain | Residue |
| A | ASN67 |
| A | THR91 |
| A | HOH2262 |
| A | HOH2378 |
| A | HOH2400 |
| A | HOH2449 |
| site_id | AD1 |
| Number of Residues | 6 |
| Details | binding site for residue NA A 1010 |
| Chain | Residue |
| A | ASN511 |
| A | THR513 |
| A | GLN517 |
| A | HOH1607 |
| A | HOH2249 |
| A | HOH2586 |
| site_id | AD2 |
| Number of Residues | 6 |
| Details | binding site for residue NA A 1011 |
| Chain | Residue |
| A | ASP452 |
| A | HOH1293 |
| A | HOH1843 |
| A | HOH2225 |
| A | HOH2265 |
| A | HOH2783 |
| site_id | AD3 |
| Number of Residues | 9 |
| Details | binding site for residue MLI A 1012 |
| Chain | Residue |
| A | TYR376 |
| A | THR377 |
| A | LEU378 |
| A | TYR381 |
| A | GLU382 |
| A | ARG825 |
| A | 7ML1002 |
| A | GOL1038 |
| A | HOH1160 |
| site_id | AD4 |
| Number of Residues | 8 |
| Details | binding site for residue MLI A 1013 |
| Chain | Residue |
| A | SER205 |
| A | LYS577 |
| A | LEU628 |
| A | PHE629 |
| A | ASP630 |
| A | HOH1103 |
| A | HOH1396 |
| A | HOH2492 |
| site_id | AD5 |
| Number of Residues | 9 |
| Details | binding site for residue MLI A 1014 |
| Chain | Residue |
| A | ALA531 |
| A | LEU532 |
| A | TRP546 |
| A | LEU551 |
| A | ASP566 |
| A | ALA567 |
| A | SER570 |
| A | HOH1133 |
| A | HOH1175 |
| site_id | AD6 |
| Number of Residues | 11 |
| Details | binding site for residue MLI A 1015 |
| Chain | Residue |
| A | GLU397 |
| A | GLU398 |
| A | HOH1169 |
| A | HOH1171 |
| A | HOH1223 |
| A | HOH1432 |
| A | HOH1437 |
| A | HOH1946 |
| A | HOH1952 |
| A | HOH2539 |
| A | GLY396 |
| site_id | AD7 |
| Number of Residues | 10 |
| Details | binding site for residue MLI A 1016 |
| Chain | Residue |
| A | GLU123 |
| A | TRP313 |
| A | PHE314 |
| A | VAL369 |
| A | MET372 |
| A | HOH1145 |
| A | HOH1189 |
| A | HOH1239 |
| A | HOH1261 |
| A | HOH1719 |
| site_id | AD8 |
| Number of Residues | 4 |
| Details | binding site for residue DMS A 1017 |
| Chain | Residue |
| A | THR393 |
| A | GLU535 |
| A | HOH1214 |
| A | HOH2077 |
| site_id | AD9 |
| Number of Residues | 5 |
| Details | binding site for residue DMS A 1018 |
| Chain | Residue |
| A | GLU627 |
| A | PHE629 |
| A | ASP630 |
| A | ARG835 |
| A | HOH1119 |
| site_id | AE1 |
| Number of Residues | 6 |
| Details | binding site for residue DMS A 1019 |
| Chain | Residue |
| A | THR393 |
| A | LEU394 |
| A | LEU395 |
| A | GLY396 |
| A | ASN507 |
| A | HOH1796 |
| site_id | AE2 |
| Number of Residues | 5 |
| Details | binding site for residue DMS A 1020 |
| Chain | Residue |
| A | ASP28 |
| A | LEU29 |
| A | ASP30 |
| A | LYS33 |
| A | ARG171 |
| site_id | AE3 |
| Number of Residues | 6 |
| Details | binding site for residue DMS A 1021 |
| Chain | Residue |
| A | ARG14 |
| A | ALA15 |
| A | PRO16 |
| A | ASP17 |
| A | HOH2601 |
| A | HOH2779 |
| site_id | AE4 |
| Number of Residues | 4 |
| Details | binding site for residue CL A 1022 |
| Chain | Residue |
| A | ASP216 |
| A | ALA254 |
| A | SER273 |
| A | HOH1749 |
| site_id | AE5 |
| Number of Residues | 5 |
| Details | binding site for residue CL A 1023 |
| Chain | Residue |
| A | LEU617 |
| A | LEU619 |
| A | HOH1578 |
| A | HOH1730 |
| A | HOH1840 |
| site_id | AE6 |
| Number of Residues | 4 |
| Details | binding site for residue CL A 1024 |
| Chain | Residue |
| A | GLU800 |
| A | ASP801 |
| A | HOH1870 |
| A | HOH2464 |
| site_id | AE7 |
| Number of Residues | 4 |
| Details | binding site for residue CL A 1025 |
| Chain | Residue |
| A | ALA31 |
| A | GLN32 |
| A | HOH1426 |
| A | HOH2004 |
| site_id | AE8 |
| Number of Residues | 5 |
| Details | binding site for residue GOL A 1026 |
| Chain | Residue |
| A | GLU292 |
| A | VAL670 |
| A | HIS672 |
| A | HOH1167 |
| A | HOH1784 |
| site_id | AE9 |
| Number of Residues | 8 |
| Details | binding site for residue GOL A 1027 |
| Chain | Residue |
| A | ARG681 |
| A | GLN703 |
| A | ALA707 |
| A | ASN708 |
| A | ASN709 |
| A | ASP712 |
| A | HOH1333 |
| A | HOH1909 |
| site_id | AF1 |
| Number of Residues | 7 |
| Details | binding site for residue GOL A 1028 |
| Chain | Residue |
| A | PHE704 |
| A | LYS738 |
| A | TRP739 |
| A | HOH1141 |
| A | HOH1307 |
| A | HOH1667 |
| A | HOH2123 |
| site_id | AF2 |
| Number of Residues | 6 |
| Details | binding site for residue GOL A 1029 |
| Chain | Residue |
| A | TRP74 |
| A | LYS75 |
| A | HOH1130 |
| A | HOH1397 |
| A | HOH1585 |
| A | HOH1876 |
| site_id | AF3 |
| Number of Residues | 8 |
| Details | binding site for residue GOL A 1030 |
| Chain | Residue |
| A | LEU61 |
| A | VAL62 |
| A | TRP74 |
| A | ARG669 |
| A | GLU671 |
| A | HOH1155 |
| A | HOH1397 |
| A | HOH1973 |
| site_id | AF4 |
| Number of Residues | 6 |
| Details | binding site for residue GOL A 1031 |
| Chain | Residue |
| A | ASP58 |
| A | LEU59 |
| A | ILE98 |
| A | HOH1274 |
| A | HOH1704 |
| A | HOH1992 |
| site_id | AF5 |
| Number of Residues | 5 |
| Details | binding site for residue GOL A 1032 |
| Chain | Residue |
| A | ASP68 |
| A | GLU69 |
| A | ARG279 |
| A | ASP281 |
| A | HOH1157 |
| site_id | AF6 |
| Number of Residues | 8 |
| Details | binding site for residue GOL A 1033 |
| Chain | Residue |
| A | SER112 |
| A | ALA115 |
| A | PHE194 |
| A | ASP216 |
| A | ALA254 |
| A | VAL255 |
| A | ASP256 |
| A | HOH1158 |
| site_id | AF7 |
| Number of Residues | 10 |
| Details | binding site for residue GOL A 1034 |
| Chain | Residue |
| A | MET368 |
| A | PRO473 |
| A | ASP474 |
| A | ALA476 |
| A | HOH1122 |
| A | HOH1328 |
| A | HOH1359 |
| A | HOH1888 |
| A | HOH1919 |
| A | HOH2018 |
| site_id | AF8 |
| Number of Residues | 13 |
| Details | binding site for residue GOL A 1035 |
| Chain | Residue |
| A | HIS11 |
| A | ASP12 |
| A | ARG468 |
| A | THR469 |
| A | LYS478 |
| A | GLN479 |
| A | HOH1312 |
| A | HOH1590 |
| A | HOH1779 |
| A | HOH1788 |
| A | HOH1846 |
| A | HOH2158 |
| A | HOH2312 |
| site_id | AF9 |
| Number of Residues | 14 |
| Details | binding site for residue GOL A 1036 |
| Chain | Residue |
| A | ARG293 |
| A | VAL324 |
| A | ASP327 |
| A | GLN328 |
| A | ASN344 |
| A | GLU382 |
| A | GOL1038 |
| A | HOH1128 |
| A | HOH1160 |
| A | HOH1217 |
| A | HOH1269 |
| A | HOH1734 |
| A | HOH1823 |
| A | HOH1880 |
| site_id | AG1 |
| Number of Residues | 5 |
| Details | binding site for residue GOL A 1037 |
| Chain | Residue |
| A | ASN623 |
| A | GLY791 |
| A | HOH1129 |
| A | HOH1257 |
| A | HOH1634 |
| site_id | AG2 |
| Number of Residues | 6 |
| Details | binding site for residue GOL A 1038 |
| Chain | Residue |
| A | TYR275 |
| A | ARG293 |
| A | MLI1012 |
| A | GOL1036 |
| A | HOH1114 |
| A | HOH1160 |
| site_id | AG3 |
| Number of Residues | 10 |
| Details | binding site for residue GOL A 1039 |
| Chain | Residue |
| A | VAL622 |
| A | ALA638 |
| A | THR755 |
| A | SER792 |
| A | ASN793 |
| A | HOH1179 |
| A | HOH1488 |
| A | HOH1606 |
| A | HOH1966 |
| A | HOH2033 |
Functional Information from PROSITE/UniProt
| site_id | PS00142 |
| Number of Residues | 10 |
| Details | ZINC_PROTEASE Neutral zinc metallopeptidases, zinc-binding region signature. VIGHEYFHNW |
| Chain | Residue | Details |
| A | VAL294-TRP303 |
Functional Information from SwissProt/UniProt
| site_id | SWS_FT_FI1 |
| Number of Residues | 1 |
| Details | Active site: {"description":"Proton acceptor","evidences":[{"source":"PubMed","id":"16885166","evidenceCode":"ECO:0000305"},{"source":"PubMed","id":"18416562","evidenceCode":"ECO:0000305"},{"source":"PubMed","id":"19622865","evidenceCode":"ECO:0000305"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI2 |
| Number of Residues | 8 |
| Details | Binding site: {} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI3 |
| Number of Residues | 1 |
| Details | Site: {"description":"Transition state stabilizer","evidences":[{"evidenceCode":"ECO:0000305"}]} |
| Chain | Residue | Details |
