5MA5

GFP-binding DARPin fusion gc_K11

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	B, D	Green fluorescent protein	polymer	245	27453.9	2	UniProt (P42212) Pfam (PF01353) In PDB	Aequorea victoria (Jellyfish)
2	A, C	K11	polymer	302	31635.2	2		synthetic construct
3	B, D, C	1,2-ETHANEDIOL	non-polymer		62.1	3	Chemie (EDO)
4	A, C	CITRIC ACID	non-polymer		192.1	4	Chemie (CIT)
5	A	ISOPROPYL ALCOHOL	non-polymer		60.1	2	Chemie (IPA)
6	A, C	SODIUM ION	non-polymer		23.0	2	Chemie (NA)
7		water	water		18.0	779	Chemie (HOH)

Sequence modifications

B, D: 2 - 238 (UniProt: P42212)

PDB	External Database	Details
Gly -4	-	expression tag
Pro -3	-	expression tag
Gly -2	-	expression tag
Ser -1	-	expression tag
Met 0	-	expression tag
Val 1	-	expression tag
Leu 64	Phe 64	conflict
Cro 66	Ser 65	chromophore
Cro 66	Tyr 66	chromophore
Cro 66	Gly 67	chromophore
Leu 231	His 231	conflict
Gln 239	-	expression tag
Ala 240	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	118178.3
Non-Polymers*	Number of molecules	11
	Total formula weight	1120.9
All*	Total formula weight	119299.1

*Water molecules are not included.