5M8S

Crystal structure of human tyrosinase related protein 1 mutant (T391V-R374S-Y362F) in complex with phenylthiourea (PTU)

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D	5,6-dihydroxyindole-2-carboxylic acid oxidase	polymer	446	50531.2	4	UniProt (P17643) Pfam (PF00264) In PDB	Homo sapiens (Human)	DHICA oxidase,Catalase B,Glycoprotein 75,Melanoma antigen gp75,Tyrosinase-related protein 1,TRP1
2	E, H, P	2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-[alpha-L-fucopyranose-(1-6)]2-acetamido-2-deoxy-beta-D-glucopyranose	branched		570.5	3	In PDB GlyTouCan (G21290RB)
3	F, G, J, L, N...	2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		424.4	8	In PDB GlyTouCan (G42666HT)
4	I	alpha-D-mannopyranose-(1-6)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		383.3	1	In PDB GlyTouCan (G87612PG)
5	K	alpha-D-mannopyranose-(1-3)-[alpha-D-mannopyranose-(1-6)]alpha-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-[alpha-L-fucopyranose-(1-6)]2-acetamido-2-deoxy-beta-D-glucopyranose	branched		1057.0	1	In PDB GlyTouCan (G64181RO)
6	M	alpha-L-fucopyranose-(1-6)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		367.3	1	In PDB GlyTouCan (G86851RC)
7	Q	alpha-D-mannopyranose-(1-3)-alpha-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-[alpha-L-fucopyranose-(1-6)]2-acetamido-2-deoxy-beta-D-glucopyranose	branched		894.8	1	In PDB GlyTouCan (G26032DN)
8	S	alpha-D-mannopyranose-(1-3)-[alpha-D-mannopyranose-(1-6)]alpha-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		910.8	1	In PDB GlyTouCan (G21381MC)
9	C, A	2-acetamido-2-deoxy-beta-D-glucopyranose	non-polymer		221.2	4	Chemie (NAG)
10	C, D, A, B	N-PHENYLTHIOUREA	non-polymer		152.2	4	Chemie (URS)
11	B, C, D, A	ZINC ION	non-polymer		65.4	9	Chemie (ZN)
12		water	water		18.0	722	Chemie (HOH)

Sequence modifications

A, B, C, D: 25 - 470 (UniProt: P17643)

PDB	External Database	Details
Phe 362	Tyr 362	engineered mutation
Ser 374	Arg 374	engineered mutation
Val 391	Thr 391	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	202124.6
Branched	Number of molecules	16
	Total formula weight	8720.1
Non-Polymers*	Number of molecules	17
	Total formula weight	2082.4
All*	Total formula weight	212927.2

*Water molecules are not included.