5M8S
Crystal structure of human tyrosinase related protein 1 mutant (T391V-R374S-Y362F) in complex with phenylthiourea (PTU)
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B, C, D (A, B, C, D) | 5,6-dihydroxyindole-2-carboxylic acid oxidase | polymer | 446 | 50531.2 | 4 | UniProt (P17643) Pfam (PF00264) | Homo sapiens (Human) | DHICA oxidase,Catalase B,Glycoprotein 75,Melanoma antigen gp75,Tyrosinase-related protein 1,TRP1 |
| 2 | E, H, P (E, H, P) | 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-[alpha-L-fucopyranose-(1-6)]2-acetamido-2-deoxy-beta-D-glucopyranose | branched | 570.5 | 3 | In PDB GlyTouCan (G21290RB) | |||
| 3 | F, G, J, L, N... (F, G, J, L, N...) | 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose | branched | 424.4 | 8 | In PDB GlyTouCan (G42666HT) | |||
| 4 | I (I) | alpha-D-mannopyranose-(1-6)-2-acetamido-2-deoxy-beta-D-glucopyranose | branched | 383.3 | 1 | In PDB GlyTouCan (G87612PG) | |||
| 5 | K (K) | alpha-D-mannopyranose-(1-3)-[alpha-D-mannopyranose-(1-6)]alpha-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-[alpha-L-fucopyranose-(1-6)]2-acetamido-2-deoxy-beta-D-glucopyranose | branched | 1057.0 | 1 | In PDB GlyTouCan (G64181RO) | |||
| 6 | M (M) | alpha-L-fucopyranose-(1-6)-2-acetamido-2-deoxy-beta-D-glucopyranose | branched | 367.3 | 1 | In PDB GlyTouCan (G86851RC) | |||
| 7 | Q (Q) | alpha-D-mannopyranose-(1-3)-alpha-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-[alpha-L-fucopyranose-(1-6)]2-acetamido-2-deoxy-beta-D-glucopyranose | branched | 894.8 | 1 | In PDB GlyTouCan (G26032DN) | |||
| 8 | S (S) | alpha-D-mannopyranose-(1-3)-[alpha-D-mannopyranose-(1-6)]alpha-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose | branched | 910.8 | 1 | In PDB GlyTouCan (G21381MC) | |||
| 9 | CA, DA, EA, U (C, A) | 2-acetamido-2-deoxy-beta-D-glucopyranose | non-polymer | 221.2 | 4 | Chemie (NAG) | |||
| 10 | FA, IA, V, Z (C, D, A, B) | N-PHENYLTHIOUREA | non-polymer | 152.2 | 4 | Chemie (URS) | |||
| 11 | AA, BA, GA, HA, JA... (B, C, D, A) | ZINC ION | non-polymer | 65.4 | 9 | Chemie (ZN) | |||
| 12 | LA, MA, NA, OA (A, B, C, D) | water | water | 18.0 | 722 | Chemie (HOH) |
Sequence modifications
A, B, C, D: 25 - 470 (UniProt: P17643)
| PDB | External Database | Details |
|---|---|---|
| Phe 362 | Tyr 362 | engineered mutation |
| Ser 374 | Arg 374 | engineered mutation |
| Val 391 | Thr 391 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 4 |
| Total formula weight | 202124.6 | |
| Branched | Number of molecules | 16 |
| Total formula weight | 8720.1 | |
| Non-Polymers* | Number of molecules | 17 |
| Total formula weight | 2082.4 | |
| All* | Total formula weight | 212927.2 |
