5M8S

Crystal structure of human tyrosinase related protein 1 mutant (T391V-R374S-Y362F) in complex with phenylthiourea (PTU)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE MASSIF-1
Synchrotron site	ESRF
Beamline	MASSIF-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-02-01
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.976
Spacegroup name	P 21 21 21
Unit cell lengths	90.081, 141.767, 191.730
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	45.883 - 2.200
R-factor	0.1957
Rwork	0.194
R-free	0.23490
Data scaling software	SCALA
Phasing software	PHENIX
Refinement software	REFMAC

Data quality characteristics

	Overall
Low resolution limit [Å]	48.920
High resolution limit [Å]	2.200
Number of reflections	124119
<I/σ(I)>	10
Completeness [%]	99.4
Redundancy	4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		290	0.1 M Tris (pH 7.0), 0.2 M NaCl and 30% (w/v) PEG 3000