5M8S
Crystal structure of human tyrosinase related protein 1 mutant (T391V-R374S-Y362F) in complex with phenylthiourea (PTU)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE MASSIF-1 |
Synchrotron site | ESRF |
Beamline | MASSIF-1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-02-01 |
Detector | DECTRIS PILATUS 6M-F |
Wavelength(s) | 0.976 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 90.081, 141.767, 191.730 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 45.883 - 2.200 |
R-factor | 0.1957 |
Rwork | 0.194 |
R-free | 0.23490 |
Data scaling software | SCALA |
Phasing software | PHENIX |
Refinement software | REFMAC |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 48.920 |
High resolution limit [Å] | 2.200 |
Number of reflections | 124119 |
<I/σ(I)> | 10 |
Completeness [%] | 99.4 |
Redundancy | 4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 290 | 0.1 M Tris (pH 7.0), 0.2 M NaCl and 30% (w/v) PEG 3000 |