5M73

Structure of the human SRP S domain with SRP72 RNA-binding domain

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, E	Human gene for small cytoplasmic 7SL RNA (7L30.1)	polymer	145	47200.0	2	GenBank (23924)	Homo sapiens
2	B, F	Signal recognition particle 19 kDa protein	polymer	128	14791.1	2	UniProt (P09132) Pfam (PF01922) In PDB	Homo sapiens (Human)	SRP19
3	C, G	Signal recognition particle subunit SRP68	polymer	203	24280.8	2	UniProt (Q9UHB9) Pfam (PF16969) In PDB	Homo sapiens (Human)	SRP68,Signal recognition particle 68 kDa protein
4	D, H	Signal recognition particle subunit SRP72	polymer	158	17189.6	2	UniProt (O76094) Pfam (PF08492) In PDB	Homo sapiens (Human)	SRP72,Signal recognition particle 72 kDa protein
5	A, E, G, C	MAGNESIUM ION	non-polymer		24.3	45	Chemie (MG)
6	A, E	POTASSIUM ION	non-polymer		39.1	10	Chemie (K)
7	A, E, B	GLYCEROL	non-polymer		92.1	9	Chemie (GOL)

Sequence modifications

A, E: 106 - 248 (GenBank: 23924)

PDB	External Database	Details
Gtp 105	-	insertion
A 249	-	insertion

B, F: 11 - 118 (UniProt: P09132)

PDB	External Database	Details
Met 1	-	initiating methionine
Ala 2	-	expression tag
Cys 3	-	expression tag
Ala 4	-	expression tag
Ala 5	-	expression tag
Ala 6	-	expression tag
Arg 7	-	expression tag
Ser 8	-	expression tag
Pro 9	-	expression tag
Ala 10	-	expression tag
Thr 119	-	expression tag
Gln 120	-	expression tag
Leu 121	-	expression tag
Glu 122	-	expression tag
His 123	-	expression tag
His 124	-	expression tag
His 125	-	expression tag
His 126	-	expression tag
His 127	-	expression tag
His 128	-	expression tag

C, G: 60 - 254 (UniProt: Q9UHB9)

PDB	External Database	Details
Met 52	-	initiating methionine
Gly 53	-	expression tag
His 54	-	expression tag
His 55	-	expression tag
His 56	-	expression tag
His 57	-	expression tag
His 58	-	expression tag
His 59	-	expression tag
Asp 116	Glu 116	conflict

D, H: 512 - 668 (UniProt: O76094)

PDB	External Database	Details
Met 511	-	initiating methionine
Gly 512	Met 512	conflict
His 663	Lys 663	conflict
His 664	Lys 664	conflict
His 665	Lys 665	conflict
His 666	Gly 666	conflict
His 667	Gly 667	conflict
His 668	Lys 668	conflict

Contents of the asymmetric unit

Polymers	Number of chains	8
	Total formula weight	206923.0
Non-Polymers*	Number of molecules	64
	Total formula weight	2313.6
All*	Total formula weight	209236.5

*Water molecules are not included.