5M73

Structure of the human SRP S domain with SRP72 RNA-binding domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID29
Synchrotron site	ESRF
Beamline	ID29
Temperature [K]	80
Detector technology	PIXEL
Collection date	2015-02-06
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.97779
Spacegroup name	P 21 21 21
Unit cell lengths	134.567, 139.306, 152.246
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.390 - 3.400
R-factor	0.239
Rwork	0.237
R-free	0.28000
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4p3e
RMSD bond length	0.010
RMSD bond angle	1.567
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.10.1_2155)

Data quality characteristics

	Overall
Low resolution limit [Å]	48.400
High resolution limit [Å]	3.400
Rmerge	0.253
Number of reflections	39893
<I/σ(I)>	8.7
Completeness [%]	99.7
Redundancy	13.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		277.15	17 % (w/v) PEG3350 0.1 M KF 0.1 M Tris pH 8.5