5M5N

Pseudo-atomic model of microtubule-bound S.pombe kinesin-5 motor domain in the AMPPNP state (based on cryo-electron microscopy experiment): the N-terminus adopts multiple conformations.

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	Tubulin alpha-1D chain	polymer	452	50236.4	1	UniProt (Q2HJ86) Pfam (PF00091) Pfam (PF03953) In PDB	Bos taurus (Bovine)
2	B	Tubulin beta-2B chain	polymer	445	49907.8	1	UniProt (Q6B856) Pfam (PF00091) Pfam (PF03953) In PDB	Bos taurus (Bovine)
3	C	Kinesin-like protein cut7	polymer	369	40737.5	1	UniProt (P24339) Pfam (PF00225) In PDB	Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)	Cell untimely torn protein 7
4	A, C	MAGNESIUM ION	non-polymer		24.3	2	Chemie (MG)
5	A	GUANOSINE-5'-TRIPHOSPHATE	non-polymer		523.2	1	Chemie (GTP)
6	B	GUANOSINE-5'-DIPHOSPHATE	non-polymer		443.2	1	Chemie (GDP)
7	B	TAXOL	non-polymer		853.9	1	Chemie (TA1)
8	C	PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER	non-polymer		506.2	1	Chemie (ANP)

Sequence modifications

A: 1 - 452 (UniProt: Q2HJ86)

PDB	External Database	Details
Ile 7	Val 7	conflict
Ile 114	Leu 114	conflict
Ser 136	Leu 136	conflict
Val 137	Ile 137	conflict
Gly 265	Ile 265	conflict
Glu 358	Gln 358	conflict
Val 437	Met 437	conflict
Glu 450	Asp 450	conflict

B: 1 - 445 (UniProt: Q6B856)

PDB	External Database	Details
Ala 55	Thr 55	conflict
Val 170	Met 170	conflict
Ala 296	Ser 296	conflict
Val 316	Ile 316	conflict

Contents of the asymmetric unit

Polymers	Number of chains	3
	Total formula weight	140881.6
Non-Polymers*	Number of molecules	6
	Total formula weight	2375.1
All*	Total formula weight	143256.7

*Water molecules are not included.