5M5N
Pseudo-atomic model of microtubule-bound S.pombe kinesin-5 motor domain in the AMPPNP state (based on cryo-electron microscopy experiment): the N-terminus adopts multiple conformations.
EM specimen
Vitrification | Yes |
Instrument | FEI VITROBOT MARK I |
EM Data Collection
Microscope | FEI TECNAI F20 |
Electron Gun | FIELD EMISSION GUN |
Accelerating Voltage (kV) | 200 |
Detector | GATAN ULTRASCAN 4000 (4k x 4k) |
3D Reconstruction
Method | SINGLE PARTICLE |
Resolution (Å) | 9.3 |
Software | EMAN,SPIDER,FREALIGN,Flex-EM,MODELLER,CTFFIND,UCSF Chimera |
CTF correction | PHASE FLIPPING AND AMPLITUDE CORRECTION |