5M2Q
Structure of cobinamide-bound BtuF mutant W66F, the periplasmic vitamin B12 binding protein in E.coli
Entity
Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
1 | A, B | Outer membrane protein A,Vitamin B12-binding protein | polymer | 289 | 31472.9 | 2 | UniProt (P0A910) UniProt (P37028) Pfam (PF01497) In PDB | Escherichia coli | Outer membrane protein II* |
2 | A, B | COB(II)INAMIDE | non-polymer | 990.1 | 2 | Chemie (CBY) | |||
3 | A, B | CYANIDE ION | non-polymer | 26.0 | 2 | Chemie (CYN) | |||
4 | A, B | GLYCEROL | non-polymer | 92.1 | 3 | Chemie (GOL) | |||
5 | water | water | 18.0 | 390 | Chemie (HOH) |
Sequence modifications
A, B: -2 - 19 (UniProt: P0A910)
A, B: 22 - 266 (UniProt: P37028)
PDB | External Database | Details |
---|---|---|
Ser 20 | - | linker |
Met 21 | - | linker |
PDB | External Database | Details |
---|---|---|
Phe 66 | Trp 66 | engineered mutation |
Ser 267 | - | expression tag |
Gly 268 | - | expression tag |
Ser 269 | - | expression tag |
Leu 270 | - | expression tag |
Glu 271 | - | expression tag |
Val 272 | - | expression tag |
Leu 273 | - | expression tag |
Phe 274 | - | expression tag |
Gln 275 | - | expression tag |
Gly 276 | - | expression tag |
Pro 277 | - | expression tag |
Gly 278 | - | expression tag |
Gly 279 | - | expression tag |
Ser 280 | - | expression tag |
His 281 | - | expression tag |
His 282 | - | expression tag |
His 283 | - | expression tag |
His 284 | - | expression tag |
His 285 | - | expression tag |
His 286 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
Polymers | Number of chains | 2 |
Total formula weight | 62945.8 | |
Non-Polymers* | Number of molecules | 7 |
Total formula weight | 2308.5 | |
All* | Total formula weight | 65254.3 |