5M2Q
Structure of cobinamide-bound BtuF mutant W66F, the periplasmic vitamin B12 binding protein in E.coli
Functional Information from GO Data
Functional Information from PDB Data
| site_id | AC1 |
| Number of Residues | 20 |
| Details | binding site for residue CBY A 301 |
| Chain | Residue |
| A | PRO31 |
| A | SER241 |
| A | ASP242 |
| A | GLU245 |
| A | ARG246 |
| A | CYN302 |
| A | HOH410 |
| A | HOH413 |
| A | HOH415 |
| A | HOH457 |
| A | HOH463 |
| A | ALA32 |
| A | HOH550 |
| A | TYR50 |
| A | PHE66 |
| A | TRP85 |
| A | GLY87 |
| A | PHE162 |
| A | PHE168 |
| A | TRP196 |
| site_id | AC2 |
| Number of Residues | 4 |
| Details | binding site for residue CYN A 302 |
| Chain | Residue |
| A | GLU245 |
| A | CBY301 |
| A | HOH415 |
| A | HOH530 |
| site_id | AC3 |
| Number of Residues | 6 |
| Details | binding site for residue GOL A 303 |
| Chain | Residue |
| A | MET105 |
| A | TRP106 |
| A | HOH437 |
| B | VAL104 |
| B | TRP106 |
| B | GOL303 |
| site_id | AC4 |
| Number of Residues | 2 |
| Details | binding site for residue GOL A 304 |
| Chain | Residue |
| A | ARG25 |
| A | VAL45 |
| site_id | AC5 |
| Number of Residues | 19 |
| Details | binding site for residue CBY B 301 |
| Chain | Residue |
| B | PRO31 |
| B | ALA32 |
| B | TYR50 |
| B | PHE66 |
| B | GLN67 |
| B | TRP85 |
| B | GLY87 |
| B | PHE162 |
| B | PHE168 |
| B | ASP242 |
| B | GLU245 |
| B | ARG246 |
| B | CYN302 |
| B | HOH406 |
| B | HOH422 |
| B | HOH467 |
| B | HOH471 |
| B | HOH510 |
| B | HOH529 |
| site_id | AC6 |
| Number of Residues | 2 |
| Details | binding site for residue CYN B 302 |
| Chain | Residue |
| B | GLU245 |
| B | CBY301 |
| site_id | AC7 |
| Number of Residues | 10 |
| Details | binding site for residue GOL B 303 |
| Chain | Residue |
| A | ARG86 |
| A | TRP106 |
| A | GOL303 |
| B | TRP106 |
| B | VAL107 |
| B | ASP108 |
| B | ALA109 |
| B | ALA118 |
| B | HOH478 |
| B | HOH501 |
Functional Information from SwissProt/UniProt
| site_id | SWS_FT_FI1 |
| Number of Residues | 2 |
| Details | BINDING: BINDING => ECO:0000255|HAMAP-Rule:MF_01000, ECO:0000269|PubMed:12468528, ECO:0007744|PDB:1N4A |
| Chain | Residue | Details |
| A | TYR50 | |
| B | TYR50 |
| site_id | SWS_FT_FI2 |
| Number of Residues | 2 |
| Details | BINDING: BINDING => ECO:0000255|HAMAP-Rule:MF_01000, ECO:0000269|PubMed:12468528, ECO:0000269|PubMed:12475936, ECO:0007744|PDB:1N2Z, ECO:0007744|PDB:1N4A |
| Chain | Residue | Details |
| A | ASP242 | |
| B | ASP242 |
| site_id | SWS_FT_FI3 |
| Number of Residues | 4 |
| Details | SITE: Important for BtuC binding => ECO:0000255|HAMAP-Rule:MF_01000, ECO:0000305|PubMed:12475936 |
| Chain | Residue | Details |
| A | GLU72 | |
| A | GLU202 | |
| B | GLU72 | |
| B | GLU202 |
