5M1E

Crystal structure of N-terminally tagged UbiD from E. coli reconstituted with prFMN cofactor

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C (A, B, C)	3-octaprenyl-4-hydroxybenzoate carboxy-lyase	polymer	517	57836.8	3	UniProt (P0AAB5) Pfam (PF20695) Pfam (PF01977) Pfam (PF20696)	Escherichia coli O6:H1 (strain CFT073 / ATCC 700928 / UPEC)	Polyprenyl p-hydroxybenzoate decarboxylase
2	D, G, J (A, B, C)	MANGANESE (II) ION	non-polymer		54.9	3	Chemie (MN)
3	E, H, K (A, B, C)	(16~{R})-11,12,14,14-tetramethyl-3,5-bis(oxidanylidene)-8-[(2~{S},3~{S},4~{R})-2,3,4-tris(oxidanyl)-5-phosphonooxy-pentyl]-1,4,6,8-tetrazatetracyclo[7.7.1.0^{2,7}.0^{13,17}]heptadeca-2(7),9(17),10,12-tetraene-16-sulfonic acid	non-polymer		606.5	3	Chemie (7D9)
4	F, I, L (A, B, C)	SODIUM ION	non-polymer		23.0	3	Chemie (NA)
5	M, N, O (A, B, C)	water	water		18.0	130	Chemie (HOH)

Sequence modifications

A, B, C: 1 - 497 (UniProt: P0AAB5)

PDB	External Database	Details
Met -19	-	initiating methionine
Gly -18	-	expression tag
Ser -17	-	expression tag
Ser -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
His -10	-	expression tag
Ser -9	-	expression tag
Ser -8	-	expression tag
Gly -7	-	expression tag
Leu -6	-	expression tag
Val -5	-	expression tag
Pro -4	-	expression tag
Arg -3	-	expression tag
Gly -2	-	expression tag
Ser -1	-	expression tag
His 0	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	3
	Total formula weight	173510.5
Non-Polymers*	Number of molecules	9
	Total formula weight	2053.4
All*	Total formula weight	175563.9

*Water molecules are not included.