5M1E
Crystal structure of N-terminally tagged UbiD from E. coli reconstituted with prFMN cofactor
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-01-31 |
| Detector | DECTRIS PILATUS 2M |
| Wavelength(s) | 1.044 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 194.520, 194.520, 107.410 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 94.030 - 2.620 |
| R-factor | 0.17723 |
| Rwork | 0.175 |
| R-free | 0.21182 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 5m1c |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.731 |
| Data reduction software | xia2 |
| Data scaling software | xia2 |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 94.030 | 2.690 |
| High resolution limit [Å] | 2.620 | 2.620 |
| Rmerge | 0.083 | 1.080 |
| Number of reflections | 58691 | |
| <I/σ(I)> | 16.7 | 1.9 |
| Completeness [%] | 99.5 | 100 |
| Redundancy | 6.7 | 6.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 294 | 0.1 mM SPG pH 8, 25% w/v PEG 1500 |
