5LXM
Crystal structure of Aurora-A bound to a hydrocarbon-stapled proteomimetic of TPX2
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | Aurora kinase A | polymer | 283 | 32836.5 | 1 | UniProt (O14965) Pfam (PF00069) | Homo sapiens (human) | Aurora 2,Aurora/IPL1-related kinase 1,hARK1,Breast tumor-amplified kinase,Serine/threonine-protein kinase 15,Serine/threonine-protein kinase 6,Serine/threonine-protein kinase aurora-A |
| 2 | B (D) | Targeting protein for Xklp2 | polymer | 39 | 4362.5 | 1 | UniProt (Q9ULW0) Pfam (PF09041) | Homo sapiens | Differentially expressed in cancerous and non-cancerous lung cells 2,DIL-2,Hepatocellular carcinoma-associated antigen 519,Hepatocellular carcinoma-associated antigen 90,Protein fls353,Restricted expression proliferation-associated protein 100,p100 |
| 3 | C (A) | ADENOSINE-5'-DIPHOSPHATE | non-polymer | 427.2 | 1 | Chemie (ADP) | |||
| 4 | D (A) | TETRAETHYLENE GLYCOL | non-polymer | 194.2 | 1 | Chemie (PG4) | |||
| 5 | E (A) | SULFATE ION | non-polymer | 96.1 | 1 | Chemie (SO4) | |||
| 6 | F, G (A) | DI(HYDROXYETHYL)ETHER | non-polymer | 106.1 | 2 | Chemie (PEG) | |||
| 7 | H (A) | 2-(N-MORPHOLINO)-ETHANESULFONIC ACID | non-polymer | 195.2 | 1 | Chemie (MES) | |||
| 8 | I (A) | 1-ETHOXY-2-(2-ETHOXYETHOXY)ETHANE | non-polymer | 162.2 | 1 | Chemie (P4G) | |||
| 9 | J (A) | CHLORIDE ION | non-polymer | 35.5 | 1 | Chemie (CL) | |||
| 10 | K, L (A) | MAGNESIUM ION | non-polymer | 24.3 | 2 | Chemie (MG) | |||
| 11 | M, N (A, D) | water | water | 18.0 | 77 | Chemie (HOH) |
Sequence modifications
A: 122 - 403 (UniProt: O14965)
D: 6 - 43 (UniProt: Q9ULW0)
| PDB | External Database | Details |
|---|---|---|
| Met 121 | - | initiating methionine |
| Ala 290 | Cys 290 | engineered mutation |
| Ala 393 | Cys 393 | engineered mutation |
| PDB | External Database | Details |
|---|---|---|
| Mk8 37 | Glu 37 | conflict |
| Nh2 44 | - | amidation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 2 |
| Total formula weight | 37199.0 | |
| Non-Polymers* | Number of molecules | 10 |
| Total formula weight | 1371.3 | |
| All* | Total formula weight | 38570.3 |
