5LXM
Crystal structure of Aurora-A bound to a hydrocarbon-stapled proteomimetic of TPX2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-03-04 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.9795 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 79.780, 79.780, 140.260 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 61.980 - 2.080 |
| R-factor | 0.2018 |
| Rwork | 0.200 |
| R-free | 0.24290 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4ceg |
| Data reduction software | xia2 |
| Data scaling software | xia2 |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 61.980 |
| High resolution limit [Å] | 2.080 |
| Number of reflections | 31554 |
| <I/σ(I)> | 9.94 |
| Completeness [%] | 99.4 |
| Redundancy | 9.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 295 | MORPHEUS Crystallization Screen condition C1 |
