5LXM
Crystal structure of Aurora-A bound to a hydrocarbon-stapled proteomimetic of TPX2
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I04 |
Synchrotron site | Diamond |
Beamline | I04 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-03-04 |
Detector | DECTRIS PILATUS 6M-F |
Wavelength(s) | 0.9795 |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 79.780, 79.780, 140.260 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 61.980 - 2.080 |
R-factor | 0.2018 |
Rwork | 0.200 |
R-free | 0.24290 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4ceg |
Data reduction software | xia2 |
Data scaling software | xia2 |
Refinement software | PHENIX |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 61.980 |
High resolution limit [Å] | 2.080 |
Number of reflections | 31554 |
<I/σ(I)> | 9.94 |
Completeness [%] | 99.4 |
Redundancy | 9.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 295 | MORPHEUS Crystallization Screen condition C1 |