5LDD

Crystal structure of the heterodimeric GEF Mon1-Ccz1 in complex with Ypt7

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, D	Mon1	polymer	166	18259.0	2	UniProt (G0SGS3) Pfam (PF19036) In PDB	Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719)
2	B, E	Ccz1	polymer	250	27657.1	2	UniProt (G0SD94) Pfam (PF19031) In PDB	Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719)
3	C, F	Rab small monomeric GTPase-like protein	polymer	207	23218.2	2	UniProt (G0SGE1) Pfam (PF00071) In PDB	Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719)
4	C, F	SULFATE ION	non-polymer		96.1	3	Chemie (SO4)
5		water	water		18.0	122	Chemie (HOH)

Sequence modifications

A, D: 195 - 355 (UniProt: G0SGS3)

PDB	External Database	Details
Gly 190	-	expression tag
Pro 191	-	expression tag
Leu 192	-	expression tag
Gly 193	-	expression tag
Ser 194	-	expression tag

B, E: 1 - 249 (UniProt: G0SD94)

PDB	External Database	Details
Ser 0	-	expression tag

C, F: 1 - 205 (UniProt: G0SGE1)

PDB	External Database	Details
Gly -1	-	expression tag
Ser 0	-	expression tag
Ile 125	Asn 125	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	6
	Total formula weight	138268.7
Non-Polymers*	Number of molecules	3
	Total formula weight	288.2
All*	Total formula weight	138556.9

*Water molecules are not included.