5LDD

Crystal structure of the heterodimeric GEF Mon1-Ccz1 in complex with Ypt7

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, EMBL c/o DESY BEAMLINE P13 (MX1)
Synchrotron site	PETRA III, EMBL c/o DESY
Beamline	P13 (MX1)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-10-30
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97951
Spacegroup name	P 21 21 21
Unit cell lengths	70.083, 103.812, 206.667
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.380 - 2.496
R-factor	0.2016
Rwork	0.200
R-free	0.24110
Structure solution method	SAD
RMSD bond length	0.004
RMSD bond angle	0.620
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHENIX
Refinement software	PHENIX (dev_2439)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.380	2.650
High resolution limit [Å]	2.490	2.490
Rmeas	0.076	0.909
Number of reflections	53101
<I/σ(I)>	15.24	2.62
Completeness [%]	99.5	98.1
Redundancy	6.53

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	285.15	0.15 M ammonium sulfate, 0.1 M MES, pH 6.0, and 18% PEG 4000, 25% glycerol