5LD2
Cryo-EM structure of RecBCD+DNA complex revealing activated nuclease domain
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (B) | RecBCD enzyme subunit RecB,RecBCD enzyme subunit RecB,RecBCD enzyme subunit RecB | polymer | 1181 | 133717.4 | 1 | UniProt (P08394) Pfam (PF00580) Pfam (PF13361) Pfam (PF12705) | Escherichia coli (strain K12) | Exodeoxyribonuclease V 135 kDa polypeptide,Exodeoxyribonuclease V beta chain,Exonuclease V subunit RecB,ExoV subunit RecB,Helicase/nuclease RecBCD subunit RecB,Exodeoxyribonuclease V 135 kDa polypeptide,Exodeoxyribonuclease V beta chain,Exonuclease V subunit RecB,ExoV subunit RecB,Helicase/nuclease RecBCD subunit RecB,Exodeoxyribonuclease V 135 kDa polypeptide,Exodeoxyribonuclease V beta chain,Exonuclease V subunit RecB,ExoV subunit RecB,Helicase/nuclease RecBCD subunit RecB |
| 2 | B (C) | RecBCD enzyme subunit RecC | polymer | 1122 | 128974.1 | 1 | UniProt (P07648) Pfam (PF04257) Pfam (PF17946) | Escherichia coli (strain K12) | Exodeoxyribonuclease V 125 kDa polypeptide,Exodeoxyribonuclease V gamma chain,Exonuclease V subunit RecC,ExoV subunit RecC |
| 3 | C (D) | RecBCD enzyme subunit RecD | polymer | 609 | 67047.4 | 1 | UniProt (P04993) Pfam (PF21185) Pfam (PF13245) Pfam (PF13538) | Escherichia coli (strain K12) | Exodeoxyribonuclease V 67 kDa polypeptide,Exodeoxyribonuclease V alpha chain,Exonuclease V subunit RecD,ExoV subunit RecD,Helicase/nuclease RecBCD subunit RecD |
| 4 | D (X) | Fork-Hairpin DNA (70-MER) | polymer | 70 | 21440.7 | 1 | Endothia gyrosa | ||
| 5 | E (B) | PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER | non-polymer | 506.2 | 1 | Chemie (ANP) | |||
| 6 | F (B) | MAGNESIUM ION | non-polymer | 24.3 | 1 | Chemie (MG) |
Sequence modifications
B: 1 - 912 (UniProt: P08394)
B: 913 - 937 (PDB: 5LD2)
B: 938 - 1180 (UniProt: P08394)
D: 2 - 608 (UniProt: P04993)
| PDB | External Database | Details |
|---|---|---|
| Gly 0 | - | expression tag |
B: 938 - 1180 (UniProt: P08394)
| PDB | External Database | Details |
|---|---|---|
| Ala 1080 | Asp 1080 | engineered mutation |
| PDB | External Database | Details |
|---|---|---|
| Met 0 | - | initiating methionine |
| Gly 1 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 4 |
| Total formula weight | 351179.6 | |
| Non-Polymers* | Number of molecules | 2 |
| Total formula weight | 530.5 | |
| All* | Total formula weight | 351710.1 |
