5LD2
Cryo-EM structure of RecBCD+DNA complex revealing activated nuclease domain
EM specimen
Vitrification | Yes |
Instrument | FEI VITROBOT MARK III |
EM Data Collection
Microscope | FEI TITAN KRIOS |
Electron Gun | FIELD EMISSION GUN |
Accelerating Voltage (kV) | 300 |
Cs (mm) | 2.7 |
Energy Filter | GIF |
Detector | GATAN K2 SUMMIT (4k x 4k) |
3D Reconstruction
Method | SINGLE PARTICLE |
Resolution (Å) | 3.83 |
Software | RELION,REFMAC,PHENIX,EPU,CTFFIND,Gctf,UCSF Chimera,Coot |
CTF correction | PHASE FLIPPING AND AMPLITUDE CORRECTION |