5LBX

Structure of the T175V Etr1p mutant in the trigonal form P312 in complex with NADP and crotonyl-CoA

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B	Enoyl-[acyl-carrier-protein] reductase [NADPH, B-specific] 1, mitochondrial	polymer	385	42087.2	2	UniProt (Q8WZM3) Pfam (PF08240) Pfam (PF00107) In PDB	Candida tropicalis (Yeast)	Trans-2-enoyl-CoA reductase 1
2	A, B	NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE	non-polymer		743.4	2	Chemie (NAP)
3	A, B	SULFATE ION	non-polymer		96.1	13	Chemie (SO4)
4	A, B	CROTONYL COENZYME A	non-polymer		835.6	2	Chemie (COO)
5	A	CHLORIDE ION	non-polymer		35.5	1	Chemie (CL)
6		water	water		18.0	189	Chemie (HOH)

Sequence modifications

A, B: 23 - 386 (UniProt: Q8WZM3)

PDB	External Database	Details
Met 2	-	initiating methionine
Gly 3	-	expression tag
His 4	-	expression tag
His 5	-	expression tag
His 6	-	expression tag
His 7	-	expression tag
His 8	-	expression tag
His 9	-	expression tag
His 10	-	expression tag
His 11	-	expression tag
His 12	-	expression tag
His 13	-	expression tag
Ser 14	-	expression tag
Ser 15	-	expression tag
Gly 16	-	expression tag
His 17	-	expression tag
Ile 18	-	expression tag
Glu 19	-	expression tag
Gly 20	-	expression tag
Arg 21	-	expression tag
His 22	-	expression tag
Val 175	Thr 175	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	84174.3
Non-Polymers*	Number of molecules	18
	Total formula weight	4442.3
All*	Total formula weight	88616.6

*Water molecules are not included.