5L5U

Yeast 20S proteasome with human beta5i (1-138; V31M) and human beta6 (97-111; 118-133) in complex with epoxyketone inhibitor 17

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, O	Proteasome subunit alpha type-2	polymer	250	27191.8	2	UniProt (P23639) Pfam (PF10584) Pfam (PF00227) In PDB	Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)	Macropain subunit Y7,Multicatalytic endopeptidase complex subunit Y7,Proteasome component Y7,Proteinase YSCE subunit 7
2	B, P	Proteasome subunit alpha type-3	polymer	258	28748.2	2	UniProt (P23638) Pfam (PF10584) Pfam (PF00227) In PDB	Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)	Macropain subunit Y13,Multicatalytic endopeptidase complex subunit Y13,Proteasome component Y13,Proteinase YSCE subunit 13
3	C, Q	Proteasome subunit alpha type-4	polymer	254	28478.1	2	UniProt (P40303) Pfam (PF10584) Pfam (PF00227) In PDB	Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)	Macropain subunit PRE6,Multicatalytic endopeptidase complex subunit PRE6,Proteasome component PRE6,Proteinase YSCE subunit PRE6
4	D, R	Proteasome subunit alpha type-5	polymer	260	28649.1	2	UniProt (P32379) Pfam (PF10584) Pfam (PF00227) In PDB	Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)	Macropain subunit PUP2,Multicatalytic endopeptidase complex subunit PUP2,Proteasome component PUP2,Proteinase YSCE subunit PUP2
5	E, S	Proteasome subunit alpha type-6	polymer	234	25634.0	2	UniProt (P40302) Pfam (PF00227) Pfam (PF10584) In PDB	Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)	Macropain subunit PRE5,Multicatalytic endopeptidase complex subunit PRE5,Proteasome component PRE5,Proteinase YSCE subunit PRE5
6	F, T	Probable proteasome subunit alpha type-7	polymer	288	31575.1	2	UniProt (P21242) Pfam (PF10584) Pfam (PF00227) In PDB	Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)	Macropain subunit C1,Multicatalytic endopeptidase complex subunit C1,Proteasome component C1,Proteinase YSCE subunit 1
7	G, U	Proteasome subunit alpha type-1	polymer	252	28033.8	2	UniProt (P21243) Pfam (PF10584) Pfam (PF00227) In PDB	Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)	Macropain subunit C7-alpha,Multicatalytic endopeptidase complex C7,Proteasome component C7-alpha,Proteasome component Y8,Proteinase YSCE subunit 7,SCL1 suppressor protein
8	H, V	Proteasome subunit beta type-2	polymer	232	25114.5	2	UniProt (P25043) Pfam (PF00227) Pfam (PF12465) In PDB	Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)	Macropain subunit PUP1,Multicatalytic endopeptidase complex subunit PUP1,Proteasome component PUP1,Proteinase YSCE subunit PUP1
9	I, W	Proteasome subunit beta type-3	polymer	205	22627.8	2	UniProt (P25451) Pfam (PF00227) In PDB	Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)	Macropain subunit PUP3,Multicatalytic endopeptidase complex subunit PUP3,Proteasome component PUP3
10	J, X	Proteasome subunit beta type-4	polymer	198	22545.7	2	UniProt (P22141) Pfam (PF00227) In PDB	Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)	Macropain subunit C11,Multicatalytic endopeptidase complex subunit C11,Proteasome component C11,Proteinase YSCE subunit 11
11	K, Y	Proteasome subunit beta type-8,Proteasome subunit beta type-5	polymer	211	23394.4	2	UniProt (P28062) UniProt (P30656) Pfam (PF00227) In PDB	Homo sapiens (Human)	Low molecular mass protein 7,Macropain subunit C13,Multicatalytic endopeptidase complex subunit C13,Proteasome component C13,Proteasome subunit beta-5i,Really interesting new gene 10 protein,Macropain subunit PRE2,Multicatalytic endopeptidase complex subunit PRE2,Proteasome component PRE2,Proteinase YSCE subunit PRE2
12	L, Z	Proteasome subunit beta type-6,Proteasome subunit beta type-1,Proteasome subunit beta type-6,Proteasome subunit beta type-1,Proteasome subunit beta type-6	polymer	222	24979.0	2	UniProt (P23724) UniProt (P20618) Pfam (PF00227) In PDB	Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)	Multicatalytic endopeptidase complex subunit C5,Proteasome component C5,Macropain subunit C5,Multicatalytic endopeptidase complex subunit C5,Proteasome component C5,Proteasome gamma chain,Multicatalytic endopeptidase complex subunit C5,Proteasome component C5,Macropain subunit C5,Multicatalytic endopeptidase complex subunit C5,Proteasome component C5,Proteasome gamma chain,Multicatalytic endopeptidase complex subunit C5,Proteasome component C5
13	M, a	Proteasome subunit beta type-7	polymer	246	27200.9	2	UniProt (P30657) Pfam (PF00227) In PDB	Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)	Macropain subunit PRE4,Multicatalytic endopeptidase complex subunit PRE4,Proteasome component PRE4,Proteinase YSCE subunit PRE4
14	N, b	Proteasome subunit beta type-1	polymer	196	21517.2	2	UniProt (P38624) Pfam (PF00227) In PDB	Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)	Macropain subunit PRE3,Multicatalytic endopeptidase complex subunit PRE3,Proteasome component PRE3,Proteinase YSCE subunit PRE3
15	A, G, I, J, K...	MAGNESIUM ION	non-polymer		24.3	11	Chemie (MG)
16	G, U	CHLORIDE ION	non-polymer		35.5	2	Chemie (CL)
17	H, K, V, Y	1,2,4-trideoxy-4-methyl-2-{[N-(morpholin-4-ylacetyl)-L-alanyl-O-methyl-L-tyrosyl]amino}-1-phenyl-D-xylitol	non-polymer		584.7	4	BIRD (PRD_000991) Chemie (04C)
18		water	water		18.0	501	Chemie (HOH)

Sequence modifications

K, Y: 1 - 138 (UniProt: P28062)

PDB	External Database	Details
Met 31	Val 103	conflict

K, Y: 139 - 211 (UniProt: P30656)
L, Z: 1 - 96 (UniProt: P23724)
L, Z: 97 - 111 (UniProt: P20618)
L, Z: 112 - 117 (UniProt: P23724)
L, Z: 118 - 133 (UniProt: P20618)
L, Z: 134 - 222 (UniProt: P23724)

Contents of the asymmetric unit

Polymers	Number of chains	28
	Total formula weight	731379.3
Non-Polymers*	Number of molecules	17
	Total formula weight	2677.1
All*	Total formula weight	734056.4

*Water molecules are not included.