5KQ4

Crystal structure of S. pombe Dcp1/Dcp2 in complex with H. sapiens PNRC2 and synthetic cap analog

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	D, A	mRNA-decapping enzyme subunit 1	polymer	130	15335.5	2	UniProt (Q9P805) Pfam (PF06058) In PDB	Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
2	F, C	Proline-rich nuclear receptor coactivator 2	polymer	31	3365.8	2	UniProt (Q9NPJ4) Pfam (PF15365) In PDB	Homo sapiens (Human)
3	E, B	mRNA decapping complex subunit 2	polymer	249	28945.4	2	UniProt (O13828) Pfam (PF05026) Pfam (PF00293) In PDB	Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
4	E, B	[[(2~{R},3~{S},4~{R},5~{R})-5-(2-azanyl-7-methyl-6-oxidanylidene-3~{H}-purin-7-ium-9-yl)-3,4-bis(oxidanyl)oxolan-2-yl]methoxy-sulfanyl-phosphoryl] [[[(2~{R},3~{S},4~{R},5~{R})-5-(2-azanyl-7-methyl-6-oxidanylidene-3~{H}-purin-7-ium-9-yl)-3,4-bis(oxidanyl)oxolan-2-yl]methoxy-sulfanyl-phosphoryl]oxy-oxidanyl-phosphoryl] hydrogen phosphate	non-polymer		930.6	2	Chemie (6VQ)
5		water	water		18.0	27	Chemie (HOH)

Sequence modifications

D, A: 1 - 127 (UniProt: Q9P805)

PDB	External Database	Details
Gly -2	-	expression tag
Glu -1	-	expression tag
Phe 0	-	expression tag

E, B: 1 - 244 (UniProt: O13828)

PDB	External Database	Details
Gly -4	-	expression tag
Gly -3	-	expression tag
Gly -2	-	expression tag
Gly -1	-	expression tag
Ser 0	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	6
	Total formula weight	95293.2
Non-Polymers*	Number of molecules	2
	Total formula weight	1861.2
All*	Total formula weight	97154.4

*Water molecules are not included.