5KQ4

Crystal structure of S. pombe Dcp1/Dcp2 in complex with H. sapiens PNRC2 and synthetic cap analog

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.3.1
Synchrotron site	ALS
Beamline	8.3.1
Temperature [K]	100
Detector technology	CCD
Collection date	2015-10-01
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.115869
Spacegroup name	P 1 21 1
Unit cell lengths	43.067, 120.752, 91.849
Unit cell angles	90.00, 97.90, 90.00

Refinement procedure

Resolution	45.489 - 2.560
R-factor	0.2087
Rwork	0.206
R-free	0.25100
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2qkm
RMSD bond length	0.003
RMSD bond angle	0.655
Data reduction software	HKL-2000 (v708c)
Data scaling software	HKL-2000 (v708c)
Phasing software	PHASER
Refinement software	PHENIX ((1.10.1_2155: ???))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.610
High resolution limit [Å]	2.550	6.420	2.550
Rmerge	0.115	0.042	0.940
Number of reflections	29862
<I/σ(I)>	11.8
Completeness [%]	100.0	99.4	99.9
Redundancy	4	4	3.9
CC(1/2)		0.998	0.626

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	150 mM trilithium citrate, 13% PEG 3350, 0.05% mellitic acid, ~4 mM ZD3 soak